ArticleExperimental neurology2019
Mechanisms of decreased cholinergic arousal in focal seizures: In vivo whole-cell recordings from the pedunculopontine tegmental nucleus.
Article in Experimental neurology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 26 citations in OpenAlex.
- The basal ganglia and the epilepsies.Epilepsia open · 2026Review
- Lateral septal inhibition of nucleus basalis through direct and indirect pathways in focal limbic seizures.bioRxiv : the preprint server for biology · 2025Article
- Huperzine A attenuates epileptic seizures via enhancing dCA1-projecting septal cholinergic transmission.Acta pharmacologica Sinica · 2025Article
- Decreased locus coeruleus multiunit activity in a mouse model of temporal lobe seizures with impaired consciousness.Experimental neurology · 2025Article
- Subcortical Circuits Among Pedunculopontine Nucleus, Thalamus and Basal Ganglia Play Important Roles in Paroxysmal Arousal in Genetic Rat Models of Autosomal Dominant Sleep-Related Hypermotor Epilepsy.International journal of molecular sciences · 2025Article
- Slow and fast cortical cholinergic arousal is reduced in a mouse model of focal seizures with impaired consciousness.Cell reports · 2024Article
- A review of cell-type specific circuit mechanisms underlying epilepsy.Acta epileptologica · 2024Review
- Review
- A Century Searching for the Neurons Necessary for Wakefulness.Frontiers in neuroscience · 2022Review
- Arousal and Consciousness in Focal Seizures.Epilepsy currents · 2021Article
- Cortical low-frequency power correlates with behavioral impairment in animal model of focal limbic seizures.Epilepsia · 2021Article
- Cholinergic Signaling, Neural Excitability, and Epilepsy.Molecules (Basel, Switzerland) · 2021Review
- A Review of Neuronal Pathways Associated With Consciousness.The Journal of neuroscience nursing : journal of the American Association of Neuroscience Nurses · 2021Review
- Decreased Resting-State Functional Connectivity of Periaqueductal Gray in Temporal Lobe Epilepsy Comorbid With Migraine.Frontiers in neurology · 2021Article
- Comparison of anaesthetic- and seizure-induced states of unconsciousness: a narrative review.British journal of anaesthesia · 2021Review
- Article
- Up and Down States of Cortical Neurons in Focal Limbic Seizures.Cerebral cortex (New York, N.Y. : 1991) · 2020Article
- Propofol Causes Consciousness Loss by Affecting GABA-A Receptor in the Nucleus Basalis of Rats.Behavioural neurology · 2020Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Focal limbic seizures often impair consciousness/awareness with major negative impact on quality of life. Recent work has shown that limbic seizures depress brainstem arousal systems, including reduced action potential firing in a key node: cholinergic neurons of the pedunculopontine tegmental nucleus (PPT). In vivo whole-cell recordings have not previously been achieved in PPT, but are used here with the goal of elucidating the mechanisms of reduced PPT cholinergic neuronal activity. An established model of focal limbic seizures was used in rats following brief hippocampal stimulation under light anesthesia. Whole-cell in vivo recordings were obtained from PPT neurons using custom-fabricated 9-10 mm tapered patch pipettes, and cholinergic neurons were identified histologically. Average membrane potential, input resistance, membrane potential fluctuations and variance were analyzed during seizures. A subset of PPT neurons exhibited reduced firing and hyperpolarization during seizures and stained positive for choline acetyltransferase. These PPT neurons showed a mean membrane potential hyperpolarization of -3.82 mV (±0.81 SEM, P < .05) during seizures, and also showed significantly increased input resistance, fewer excitatory post-synaptic potential (EPSP)-like events (P < .05), and reduced membrane potential variance (P < .01). The combination of increased input resistance, decreased EPSP-like events and decreased variance weigh against active ictal inhibition and support withdrawal of excitatory input as the dominant mechanism of decreased activity of cholinergic neurons in the PPT. Further identifying synaptic mechanisms of depressed arousal during seizures may lead to new treatments to improve ictal and postictal cognition.
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