Evidence map›Paper›PMID 30543409›Full record

ArticleBioconjugate chemistry2019

Antibody-Linked Fluorogen-Activating Proteins for Antigen Detection and Cell Ablation.

Daniel S Ackerman, Burcin Altun, Dmytro Kolodieznyi, Marcel P Bruchez, Andrew Tsourkas, Jonathan W Jarvik

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bioconjugate chemistry, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.6field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 11 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Daniel S AckermanORCID 0000-0001-7810-9522
Burcin AltunDepartment of Bioengineering , University of Pennsylvania , Philadelphia , Pennsylvania 19104 , United States.
Dmytro Kolodieznyi
Marcel P BruchezORCID 0000-0002-7370-4848
Andrew TsourkasDepartment of Bioengineering , University of Pennsylvania , Philadelphia , Pennsylvania 19104 , United States.ORCID 0000-0001-7758-1753
Jonathan W Jarvik
Imaging Center · USUniversity of Pennsylvania · US

Funding

Targeted fluorescent indicators for endothelial physiology: Ca(II), ROS, NOR01EB017268 · NIBIB · CARNEGIE-MELLON UNIVERSITY · PI BRUCHEZ, MARCEL P, ST CROIX, CLAUDETTE MARIE · 2013 to 2016
$2.5M
ROS driven mitochondrial-telomere dysfunction during environmental stressR33ES025606 · NIEHS · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI OPRESKO, PATRICIA L, VAN HOUTEN, BENNETT · 2017 to 2019
$1.6M
Ultra-fast whole-heart CT using z-motion of the X-ray sourceR01EB007236 · NIBIB · UNIVERSITY OF UTAH · PI NOO, FREDERIC · 2008 to 2011
$1.3M
Bioconjugate technique for site-specific attachment of IgG onto nanoparticlesR21EB018863 · NIBIB · UNIVERSITY OF PENNSYLVANIA · PI TSOURKAS, ANDREW · 2014 to 2015
$440k
A facile method for producing bispecific antibodies from full-length IgGR21CA187657 · NCI · UNIVERSITY OF PENNSYLVANIA · PI TSOURKAS, ANDREW · 2014 to 2015
$383k
NCI NIH HHS R21 CA187657NIBIB NIH HHS R01 EB007236NIBIB NIH HHS R01 EB017268NIBIB NIH HHS R21 EB018863NIEHS NIH HHS R33 ES025606
6 · The paper itself

Abstract

We demonstrate selective labeling of cell surface proteins using fluorogen-activating proteins (FAPs) conjugated to standard immunoglobulins (IgGs). Conjugation was achieved with a polypeptide reagent comprised of an N-terminal photoactivatable Fc-binding domain and a C-terminal FAP domain. The resulting FAP-antibody conjugates were effective agents for protein detection and cell ablation in cultured mammalian cells and for visualizing cell-cell contacts using a tethered fluorogen assay. Because our approach allows FAP-antibody conjugates to be generated for most currently available IgGs, it should have broad utility for experimental and therapeutic applications.

Indexed as

Cell AdhesionCell LineCetuximabErb-b2 Receptor Tyrosine KinasesErbB ReceptorsFluorescent DyesHumansMembrane ProteinsPhotosensitizing AgentsRecombinant Fusion ProteinsTrastuzumabCetuximabEGFR protein, humanERBB2 protein, humanErb-b2 Receptor Tyrosine KinasesErbB ReceptorsFluorescent DyesMembrane ProteinsPhotosensitizing AgentsRecombinant Fusion ProteinsTrastuzumab

Identifiers

PMID30543409
PMCPMC7067197
OpenAlexW2903644795

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.