ArticleScientific reports2018
microRNA-29b mediates fibrotic induction of human xylosyltransferase-I in human dermal fibroblasts via the Sp1 pathway.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 18 citations in OpenAlex.
- miR-1, miR-133a, miR-29b and skeletal muscle fibrosis in chronic limb-threatening ischaemia.Scientific reports · 2024Article
- Human Xylosyltransferase I-An Important Linker between Acute Senescence and Fibrogenesis.Biomedicines · 2023Article
- Borrelia burgdorferi-mediated induction of miR146a-5p fine tunes the inflammatory response in human dermal fibroblasts.PloS one · 2023Article
- Understanding of arthrofibrosis: New explorative insights into extracellular matrix remodeling of synovial fibroblasts.PloS one · 2023Article
- First Characterization of Human Dermal Fibroblasts Showing a Decreased Xylosyltransferase-I Expression Induced by the CRISPR/Cas9 System.International journal of molecular sciences · 2022Article
- Dietary supplementation with sulforaphane ameliorates skin aging through activation of the Keap1-Nrf2 pathway.The Journal of nutritional biochemistry · 2021Article
- Identification of Putative Non-Substrate-Based XT-I Inhibitors by Natural Product Library Screening.Biomolecules · 2020Article
- Klotho is regulated by transcription factor Sp1 in renal tubular epithelial cells.BMC molecular and cell biology · 2020Article
- Activin A-Mediated Regulation of XT-I in Human Skin Fibroblasts.Biomolecules · 2020Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Diminished microRNA-29b levels have recently been revealed to provoke increased expression and accumulation of extracellular matrix molecules, such as collagens in fibrotic remodeling. Subsequently, the aim of this study was to find out whether microRNA-29b might also regulate human xylosyltransferase (XT)-I expression. XT-I has been characterized previously as a fibrosis biomarker catalyzing the key step of proteoglycan biosynthesis. While we demonstrate that XYLT1 is neither a target of microRNA-29b identified in silico nor a direct 3' untranslated region binding partner of microRNA-29b, transfection of normal human dermal fibroblasts with microRNA-29b inhibitor strongly increased XYLT1 mRNA expression and XT activity. Combined results of the target prediction analysis and additional transfection experiments pointed out that microRNA-29b exerts indirect influence on XT-I by targeting the transcription factor specificity protein 1 (Sp1). We could confirm our hypothesis due to the decrease in XYLT1 promoter activity after Sp1 binding site mutation and the approval of occupancy of these binding sites by Sp1 in vitro. Taken together, a hitherto unidentified pathway of XT-I regulation via microRNA-29b/Sp1 was determined in this study. Our observations will facilitate the understanding of complex molecular fibrotic pathways and provide new opportunities to investigate microRNA-based antifibrotic tools.
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