Evidence map›Paper›PMID 30541883›Full record

ArticleJournal of immunology (Baltimore, Md. : 1950)2019

Exploitation of Synthetic mRNA To Drive Immune Effector Cell Recruitment and Functional Reprogramming In Vivo.

Yitian Xu, Lu Huang, Jonathan L Kirschman, Daryll A Vanover, Pooja M Tiwari, Philip J Santangelo, Xiling Shen, David G Russell

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of immunology (Baltimore, Md. : 1950), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Review
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Yitian XuDepartment of Biological and Environmental Engineering, Cornell University, Ithaca, NY 14853.ORCID 0000-0002-0558-3749
Lu HuangDepartment of Microbiology and Immunology, Cornell University, Ithaca, NY 14853.ORCID 0000-0002-7820-2177
Jonathan L KirschmanWallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30332.
Daryll A VanoverWallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30332.ORCID 0000-0001-8744-5923
Pooja M TiwariWallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30332.ORCID 0000-0001-8170-3602
Philip J SantangeloWallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30332.
Xiling ShenDepartment of Biological and Environmental Engineering, Cornell University, Ithaca, NY 14853.ORCID 0000-0002-4978-3531
David G RussellDepartment of Microbiology and Immunology, Cornell University, Ithaca, NY 14853; dgr8@cornell.edu.ORCID 0000-0002-9748-750X

Funding

A mechanistic understanding of tuberculosis progression through bacterial reporter strainsR01AI134183 · NIAID · CORNELL UNIVERSITY · PI RUSSELL, DAVID G · 2017 to 2021
$3.5M
How does HIV lead to increased susceptibility to tuberculosis?R01AI118582 · NIAID · CORNELL UNIVERSITY · PI RUSSELL, DAVID G · 2015 to 2019
$2.8M
NIAID NIH HHS R01 AI118582NIAID NIH HHS R01 AI134183
6 · The paper itself

Abstract

Therapeutic strategies based on in vitro-transcribed mRNA (IVT) are attractive because they avoid the permanent signature of genomic integration that is associated with DNA-based therapy and result in the transient production of proteins of interest. To date, IVT has mainly been used in vaccination protocols to generate immune responses to foreign Ags. In this "proof-of-principle" study, we explore a strategy of combinatorial IVT to recruit and reprogram immune effector cells to acquire divergent biological functions in mice in vivo. First, we demonstrate that synthetic mRNA encoding CCL3 is able to recruit murine monocytes in a nonprogrammed state, exhibiting neither bactericidal nor tissue-repairing properties. However, upon addition of either

Indexed as

Cellular ReprogrammingAnimalsCells, CulturedChemokine CCL2Chemokine CCL3HeLa CellsHumansInterferon-gammaInterleukin-4LymphocytesMacrophage ActivationMacrophagesMiceMice, Inbred C57BLMonocytesProof of Concept StudyCcl2 protein, mouseCcl3 protein, mouseChemokine CCL2Chemokine CCL3Interferon-gammaInterleukin-4RNA, Messenger

Identifiers

PMID30541883
PMCPMC6325005

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.