ArticleJournal of the American Chemical Society2019
A Probe-Enabled Approach for the Selective Isolation and Characterization of Functionally Active Subpopulations in the Gut Microbiome.
Article in Journal of the American Chemical Society, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
38 citing papers in PubMed, 73 citations in OpenAlex.
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- Obtaining complete and canonical ammonia-oxidizing bacteria through specific labeling and cell sorting.ISME communications · 2025Article
- Profiling sorghum-microbe interactions with a specialized photoaffinity probe identifies key sorgoleone binders inApplied and environmental microbiology · 2024Article
- The dynamic crosslinking between gut microbiota and inflammation during aging: reviewing the nutritional and hormetic approaches against dysbiosis and inflammaging.Biogerontology · 2024Review
- Activity-based metaproteomics driven discovery and enzymological characterization of potential α-galactosidases in the mouse gut microbiome.Communications chemistry · 2024Article
- Proximitomics by Reactive Species.ACS central science · 2024Review
- Environmental activity-based protein profiling for function-driven enzyme discovery from natural communities.Environmental microbiome · 2024Article
- RETRACTED: Synthesis and Properties of Novel Alkyl-Substituted Hexaazacyclophanes and Their Diradical DicationsMolecules (Basel, Switzerland) · 2024Article
- Chemoproteomic identification of a DPP4 homolog in Bacteroides thetaiotaomicron.Nature chemical biology · 2023Article
- Activity-based protein profiling in microbes and the gut microbiome.Current opinion in chemical biology · 2023Review
- Selection and enrichment of microbial species with an increased lignocellulolytic phenotype from a native soil microbiome by activity-based probing.ISME communications · 2023Article
- Chemoproteomic Approaches for Unraveling Prokaryotic Biology.Israel journal of chemistry · 2023Article
- Interrogating the role of the milk microbiome in mastitis in the multi-omics era.Frontiers in microbiology · 2023Review
- Bioinspired large Stokes shift small molecular dyes for biomedical fluorescence imaging.Science advances · 2022Article
- Universal activity-based labeling method for ammonia- and alkane-oxidizing bacteria.The ISME journal · 2022Article
- The promise of the gut microbiome as part of individualized treatment strategies.Nature reviews. Gastroenterology & hepatology · 2022Review
Corrections and comments
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Authors and funding
12 authors at 1 institution in 1 country.
Funding
Abstract
Commensal microorganisms in the mammalian gut play important roles in host health and physiology, but a central challenge remains in achieving a detailed mechanistic understanding of specific microbial contributions to host biochemistry. New function-based approaches are needed that analyze gut microbial function at the molecular level by coupling detection and measurements of in situ biochemical activity with identification of the responsible microbes and enzymes. We developed a platform employing β-glucuronidase selective activity-based probes to detect, isolate, and identify microbial subpopulations in the gut responsible for this xenobiotic metabolism. We find that metabolic activity of gut microbiota can be plastic and that between individuals and during perturbation, phylogenetically disparate populations can provide β-glucuronidase activity. Our work links biochemical activity with molecular-scale resolution without relying on genomic inference.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.