ArticlePloS one2018
Characterization of grapevine leafroll-associated virus 3 genetic variants and application towards RT-qPCR assay design.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
29 citing papers in PubMed, 55 citations in OpenAlex.
- Virome of the Russian Grapevine Germplasm: A Final Study and Summary.Plants (Basel, Switzerland) · 2026Article
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- Metabolic Responses of Grapevine Leaves to Grapevine Leafroll-Associated Virus 3 Infection.Metabolites · 2026Article
- Phylogenetic Analysis of Grapevine Fanleaf Virus, Grapevine Virus A, and Grapevine Leafroll-Associated Virus 3 in Kazakhstan.Microorganisms · 2025Article
- Grapevine leafroll-associated virus 3: a global threat to grapevine and wine industries but a gold mine for scientific discovery.Journal of experimental botany · 2025Review
- Assessing the efficacy of thermotherapy combined with chemotherapy or cryotherapy for the eradication of grapevine leafroll-associated virus 3.Frontiers in plant science · 2025Article
- Article
- Grapevine Virome of the Don Ampelographic Collection in Russia Has Concealed Five Novel Viruses.Viruses · 2023Article
- Article
- Article
- High-Throughput Sequencing of Grapevine in Mexico Reveals a High Incidence of Viruses including a New Member of the GenusViruses · 2023Article
- A Metagenomic Investigation of the Viruses Associated with Shiraz Disease in Australia.Viruses · 2023Article
- Grapevine Leafroll-Associated Virus 3 in Single and Mixed Infections Triggers Changes in the Oxidative Balance of Four Grapevine Varieties.International journal of molecular sciences · 2022Article
- High-Throughput Sequencing Reveals Tobacco and Tomato Ringspot Viruses in Pawpaw.Plants (Basel, Switzerland) · 2022Article
- Genetic Diversity of Viral Populations Associated withPathogens (Basel, Switzerland) · 2022Article
- Biology and Ultrastructural Characterization of Grapevine Badnavirus 1 and Grapevine Virus G.Viruses · 2022Article
- Grapevine Badnavirus 1: Detection, Genetic Diversity, and Distribution in Croatia.Plants (Basel, Switzerland) · 2022Article
- Grapevine Leafroll-Associated Virus 3 Genotype Influences Foliar Symptom Development in New Zealand Vineyards.Viruses · 2022Article
- Nuances of Responses to Two Sources of Grapevine Leafroll Disease on Pinot Noir Grown in the Field for 17 Years.Viruses · 2022Article
- Prevalence of Virus Infections and GLRaV-3 Genetic Diversity in Selected Clones of Croatian Indigenous Grapevine Cultivar Plavac Mali.Pathogens (Basel, Switzerland) · 2022Article
Corrections and comments
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Authors and funding
23 authors at 9 institutions in 5 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Grapevine leafroll-associated virus 3 (GLRaV-3) is the most widely prevalent and economically important of the complex of RNA viruses associated with grapevine leafroll disease (GLD). Phylogenetic studies have grouped GLRaV-3 isolates into nine different monophyletic groups and four supergroups, making GLRaV-3 a genetically highly diverse virus species. In addition, new divergent variants have been discovered recently around the world. Accurate identification of the virus is an essential component in the management and control of GLRaV-3; however, the diversity of GLRaV-3, coupled with the limited sequence information, have complicated the development of a reliable detection assay. In this study, GLRaV-3 sequence data available in GenBank and those generated at Foundation Plant Services, University of California-Davis, was used to develop a new RT-qPCR assay with the capacity to detect all known GLRaV-3 variants. The new assay, referred to as FPST, was challenged against samples that included plants infected with different GLRaV-3 variants and originating from 46 countries. The FPST assay detected all known GLRaV-3 variants, including the highly divergent variants, by amplifying a small highly conserved region in the 3' untranslated terminal region (UTR) of the virus genome. The reliability of the new RT-qPCR assay was confirmed by an enzyme linked immunosorbent assay (ELISA) that can detect all known GLRaV-3 variants characterized to date. Additionally, three new GLRaV-3 divergent variants, represented by four isolates, were identified using a hierarchical testing process involving the FPST assay, GLRaV-3 variant-specific assays and high-throughput sequencing analysis. These variants were distantly related to groups I, II, III, V, VI, VII and IX, but much similar to GLRaV-3 variants with no assigned group; thus, they may represent new clades. Finally, based on the phylogenetic analysis, a new GLRaV-3 subclade is proposed and named as group X.
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