ArticlePloS one2018
Development of a recombinase polymerase based isothermal amplification combined with lateral flow assay (HLB-RPA-LFA) for rapid detection of "Candidatus Liberibacter asiaticus".
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 88 citations in OpenAlex.
- Development of RT-RPA assays for rapid detection of mango-infecting viruses using high-throughput sequencing-derived genomic information.BMC plant biology · 2026Article
- From Morphology to Multi-Omics: A New Age of Fusarium Research.Pathogens (Basel, Switzerland) · 2025Review
- Duplex qPCR for detecting and differentiating porcine epidemic diarrhea virus GI and GII subtypes.Frontiers in microbiology · 2025Article
- Detection of Pan-Dermatophytes and Trichophyton rubrum Using Recombinase Polymerase Amplification-Lateral Flow Dipstick Assay.Mycopathologia · 2024Article
- Isothermal Nucleic Acid Amplification-Based Lateral Flow Testing for the Detection of Plant Viruses.International journal of molecular sciences · 2024Review
- Comparison of pre-labelled primers and nucleotides as DNA labelling method for lateral flow detection of Legionella pneumophila amplicons.Scientific reports · 2024Article
- Recombinase Polymerase Amplification Combined with Lateral Flow Dipstick Assay for the Rapid and Sensitive Detection ofInternational journal of molecular sciences · 2024Article
- Development and Application of an RPA-Based Rapid Point-of-Care Testing (POCT) Method for the Detection of Feline Panleukopenia Virus.Transboundary and emerging diseases · 2024Article
- Swift and portable detection ofFrontiers in microbiology · 2024Article
- Rapid and Sensitive Detection of ToxigenicInternational journal of molecular sciences · 2023Article
- A Field Deployable Real-Time Loop-Mediated Isothermal Amplification Targeting Five Copy nrdB Gene for the Detection of 'Candidatus Liberibacter asiaticus' in Citrus.The plant pathology journal · 2023Article
- The engineered CRISPR-Mb2Cas12a variant enables sensitive and fast nucleic acid-based pathogens diagnostics in the field.Plant biotechnology journal · 2023Article
- Rapid detection of Mucorales based on recombinase polymerase amplification and real-time PCR.Frontiers in microbiology · 2023Article
- Real-time on-site detection of the three 'Frontiers in plant science · 2023Article
- Huanglongbing Pandemic: Current Challenges and Emerging Management Strategies.Plants (Basel, Switzerland) · 2022Review
- Review
- Development of a Rapid Sex Identification Method for Newborn Pigeons Using Recombinase Polymerase Amplification and a Lateral-Flow Dipstick on Farm.Animals : an open access journal from MDPI · 2022Article
- Toward Next Generation Lateral Flow Assays: Integration of Nanomaterials.Chemical reviews · 2022Review
- Insight into melatonin-mediated response and signaling in the regulation of plant defense under biotic stress.Plant molecular biology · 2022Review
- A Comprehensive Analysis of Citrus Tristeza Variants of Bhutan and Across the World.Frontiers in microbiology · 2022Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Huanglongbing (HLB) or citrus greening is highly destructive disease that is affecting the citrus industry worldwide and it has killed millions of citrus plants globally. HLB is caused by the phloem limited, Gram negative, non-culturable, alpha-proteobacterium, 'Candidatus Liberibacter asiaticus'. Currently, polymerase chain reaction (PCR) and real time PCR have been the gold standard techniques used for detection of 'Ca. L. asiaticus'. These diagnostic methods are expensive, require well equipped laboratories, not user-friendly and not suitable for on-site detection of the pathogen. In this study, a sensitive, reliable, quick and low cost recombinase polymerase based isothermal amplification combined with lateral flow assay (HLB-RPA-LFA) technique has been developed as a diagnostic tool for detection of 'Ca. L. asiaticus'. The assay was standardized by designing the specific primer pair and probe based on the conserved 16S rRNA gene of 'Ca. L. asiaticus'. The assay was optimized for temperature and reaction time by using purified DNA and crude plant extracts and the best HLB-RPA-LFA was achieved at the isothermal temperature of 38°C for 20 to 30 min. The efficacy and sensitivity of the assay was carried out by using field grown, HLB-infected, HLB-doubtful and healthy citrus cultivars including mandarin, sweet orange cv. mosambi, and acid lime. The HLB-RPA-LFA did not show cross-reactivity with other citrus pathogens and is simple, cost-effective, rapid, user-friendly and sensitive. Thus, the HLB-RPA-LFA method has great potential to provide an improved diagnostic tool for detection of 'Ca. L. asiaticus' for the farmers, nurserymen, disease surveyors, mobile plant pathology laboratories, bud-wood certification and quarantine programs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.