ArticlePLoS genetics2018
Boundaries mediate long-distance interactions between enhancers and promoters in the Drosophila Bithorax complex.
Article in PLoS genetics, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 42 citations in OpenAlex.
- Developmental patterning of adipose tissue bybioRxiv : the preprint server for biology · 2026Article
- Initiators counteract Polycomb repression and stimulate long-range contacts between enhancers and theOpen biology · 2025Article
- Article
- lncRNA read-through regulates the BX-C insulatoreLife · 2023Article
- Boundary bypass activity in theOpen biology · 2023Article
- Transcriptional Readthrough Interrupts Boundary Function in Drosophila.International journal of molecular sciences · 2023Article
- Boundary Bypass Activity in thebioRxiv : the preprint server for biology · 2023Article
- Transcriptional read through interrupts boundary function in Drosophila.bioRxiv : the preprint server for biology · 2023Article
- Mechanisms of Interaction between Enhancers and Promoters in ThreeInternational journal of molecular sciences · 2023Review
- Phosphorylated histone variant γH2Av is associated with chromatin insulators in Drosophila.PLoS genetics · 2022Article
- Harnessing changes in open chromatin determined by ATAC-seq to generate insulin-responsive reporter constructs.BMC genomics · 2022Article
- Redundant enhancers in the iab-5 domain cooperatively activate Abd-B in the A5 and A6 abdominal segments of Drosophila.Development (Cambridge, England) · 2021Article
- Mapping of functional elements of the Fab-6 boundary involved in the regulation of the Abd-B hox gene in Drosophila melanogaster.Scientific reports · 2021Article
- Homeotic Genes: Clustering, Modularity, and Diversity.Frontiers in cell and developmental biology · 2021Review
- Insights into HP1a-Chromatin Interactions.Cells · 2020Review
- The insulator functions of theScience advances · 2020Article
- Article
- Review
- Article
- Complete reconstitution of bypass and blocking functions in a minimal artificialProceedings of the National Academy of Sciences of the United States of America · 2019Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 3 countries.
Funding
Abstract
Drosophila bithorax complex (BX-C) is one of the best model systems for studying the role of boundaries (insulators) in gene regulation. Expression of three homeotic genes, Ubx, abd-A, and Abd-B, is orchestrated by nine parasegment-specific regulatory domains. These domains are flanked by boundary elements, which function to block crosstalk between adjacent domains, ensuring that they can act autonomously. Paradoxically, seven of the BX-C regulatory domains are separated from their gene target by at least one boundary, and must "jump over" the intervening boundaries. To understand the jumping mechanism, the Mcp boundary was replaced with Fab-7 and Fab-8. Mcp is located between the iab-4 and iab-5 domains, and defines the border between the set of regulatory domains controlling abd-A and Abd-B. When Mcp is replaced by Fab-7 or Fab-8, they direct the iab-4 domain (which regulates abd-A) to inappropriately activate Abd-B in abdominal segment A4. For the Fab-8 replacement, ectopic induction was only observed when it was inserted in the same orientation as the endogenous Fab-8 boundary. A similar orientation dependence for bypass activity was observed when Fab-7 was replaced by Fab-8. Thus, boundaries perform two opposite functions in the context of BX-C-they block crosstalk between neighboring regulatory domains, but at the same time actively facilitate long distance communication between the regulatory domains and their respective target genes.
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