ArticlePloS one2018
Loop-mediated isothermal amplification (LAMP) and Polymerase Chain Reaction (PCR) as quality assurance tools for Rapid Diagnostic Test (RDT) malaria diagnosis in Northern Namibia.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
10 citing papers in PubMed, 29 citations in OpenAlex.
- Article
- Assessing the influence of rapid diagnostic test (RDT) accuracy on malaria misdiagnosis and antimalarial resistance in Nigeria.Annals of medicine and surgery (2012) · 2025Review
- Accuracy of diagnosis among clinical malaria patients: comparing microscopy, RDT and a highly sensitive quantitative PCR looking at the implications for submicroscopic infections.Malaria journal · 2023Article
- Angiogenic and angiostatic factors present in the saliva of malaria patients.Malaria journal · 2022Article
- Malaria diagnostic methods with the elimination goal in view.Parasitology research · 2022Review
- Assessing the diagnostic performance of a novel RT-PCR fluorescence method for the detection of human plasmodium species.PloS one · 2022Article
- Trends of neglectedOne health (Amsterdam, Netherlands) · 2021Article
- Malaria Rapid Diagnostic Tests: Literary Review and Recommendation for a Quality Assurance, Quality Control Algorithm.Diagnostics (Basel, Switzerland) · 2021Review
- Article
- Loop Mediated Isothermal Amplification: A Promising Tool for Screening Genetic Mutations.Molecular diagnosis & therapy · 2019Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Malaria cases sometimes go undetected using RDTs due to their inaccurate use, poor storage conditions and failure to detect low parasitaemia (<50parasites/μL). This could result in continuous transmission of malaria and sustenance of parasite reservoirs. Molecular diagnostic tools are more sensitive and specific than RDTs in the detection of plasmodium parasites. However, the Polymerase Chain Reaction (PCR) is not routinely used because equipment and reagents are expensive and requires highly skilled personnel. Loop-mediated isothermal amplification (LAMP) is a relatively new molecular diagnostic tool for malaria with all the advantages of PCR (sensitive and specific) without the mentioned disadvantages. However, it has not been evaluated extensively as a point of care diagnostic in the field. One hundred and fifteen used RDTs were collected from health facilities in Northern Namibia in a blind study and PCR and LAMP were used to determine the presence of Plasmodium DNA. The sensitivities and PPV were 40.91% and 90% respectively for RDTs, 72.73% and 100% respectively for PCR with LAMP as the golden standard. In low malaria transmission settings, LAMP can be also be considered for use as a surveillance tool to detect all sources of malaria and determine proportion of low parasitaemia infections in order to eliminate them.
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Registered trials
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