ArticleMolecular biology of the cell2019
Single-molecule dynamics of the P granule scaffold MEG-3 in the Caenorhabditis elegans zygote.
Article in Molecular biology of the cell, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 22 citations in OpenAlex.
- Exploring liquid-liquid phase separation in vitro and in vivo using multimodal nonlinear optical imaging.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2025Article
- A mechanism for MEX-5-driven disassembly of PGL-3/RNA condensates in vitro.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- How germ granules promote germ cell fate.Nature reviews. Genetics · 2024Review
- The Caenorhabditis elegans TDRD5/7-like protein, LOTR-1, interacts with the helicase ZNFX-1 to balance epigenetic signals in the germline.PLoS genetics · 2022Article
- The histone H4 lysine 20 demethylase DPY-21 regulates the dynamics of condensin DC binding.Journal of cell science · 2022Article
- Regulation of biomolecular condensates by interfacial protein clusters.Science (New York, N.Y.) · 2021Article
- Single-Molecule Tracking of Chromatin-Associated Proteins in theThe journal of physical chemistry. B · 2021Article
- Article
- Going with the flow: insights fromPhilosophical transactions of the Royal Society of London. Series B, Biological sciences · 2020Review
- Recruitment of mRNAs to P granules by condensation with intrinsically-disordered proteins.eLife · 2020Article
- PLK-1 Regulation of Asymmetric Cell Division in the EarlyFrontiers in cell and developmental biology · 2020Review
- Regulation of Germ Cell mRNPs by eIF4E:4EIP Complexes: Multiple Mechanisms, One Goal.Frontiers in cell and developmental biology · 2020Review
- Switching states: dynamic remodelling of polarity complexes as a toolkit for cell polarization.Current opinion in cell biology · 2019Review
- Membraneless organelles: P granules in Caenorhabditis elegans.Traffic (Copenhagen, Denmark) · 2019Review
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
During the asymmetric division of the Caenorhabditis elegans zygote, germ (P) granules are disassembled in the anterior cytoplasm and stabilized/assembled in the posterior cytoplasm, leading to their inheritance by the germline daughter cell. P granule segregation depends on MEG (maternal-effect germline defective)-3 and MEG-4, which are enriched in P granules and in the posterior cytoplasm surrounding P granules. Here we use single-molecule imaging and tracking to characterize the reaction/diffusion mechanisms that result in MEG-3::Halo segregation. We find that the anteriorly enriched RNA-binding proteins MEX (muscle excess)-5 and MEX-6 suppress the retention of MEG-3 in the anterior cytoplasm, leading to MEG-3 enrichment in the posterior. We provide evidence that MEX-5/6 may work in conjunction with PLK-1 kinase to suppress MEG-3 retention in the anterior. Surprisingly, we find that the retention of MEG-3::Halo in the posterior cytoplasm surrounding P granules does not appear to contribute significantly to the maintenance of P granule asymmetry. Rather, our findings suggest that the formation of the MEG-3 concentration gradient and the segregation of P granules are two parallel manifestations of MEG-3's response to upstream polarity cues.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.