ArticleACS synthetic biology2019
Less Is More: Toward a Genome-Reduced Bacillus Cell Factory for "Difficult Proteins".
Article in ACS synthetic biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 77 citations in OpenAlex.
- Exploration of Length and Hydrophobicity Requirements for Optimal α-Amylase Secretion in Bacillus subtilis Based on Rational Design.Microbial biotechnology · 2026Article
- Adaptive responses of genome-reduced Bacillus subtilis during enzyme secretion in a breathing vessel culture.Microbial cell factories · 2026Article
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- The secreted proteasesVirulence · 2025Article
- A novel signal peptide toolbox for optimized heterologous expression of yeast pheromones in Bacillus subtilis.Journal, genetic engineering & biotechnology · 2025Article
- Advances in Microbial Alkaline Proteases: Addressing Industrial Bottlenecks Through Genetic and Enzyme Engineering.Applied biochemistry and biotechnology · 2025Review
- Toward Antibody Production in Genome-MinimizedACS synthetic biology · 2025Article
- Determination of the genome-scale metabolic network ofFrontiers in bioengineering and biotechnology · 2025Article
- Plasmid-encoded phosphatase RapP enhances cell growth in non-domesticated Bacillus subtilis strains.Nature communications · 2024Article
- Coenzyme A biosynthesis inmBio · 2024Article
- Metabolic Profile of the Genome-ReducedACS synthetic biology · 2024Article
- From the outer space to the inner cell: deconvoluting the complexity ofMicrobiology spectrum · 2024Article
- Bacillus and Streptomyces spp. as hosts for production of industrially relevant enzymes.Applied microbiology and biotechnology · 2024Review
- Promising non-model microbial cell factories obtained by genome reduction.Frontiers in bioengineering and biotechnology · 2024Review
- Let There Be Light: Genome Reduction EnablesACS synthetic biology · 2023Article
- Secretion-Catalyzed Assembly of Protein Biomaterials on a Bacterial Membrane Surface.Angewandte Chemie (International ed. in English) · 2023Article
- Review
- Bacillus subtilis, a Swiss Army Knife in Science and Biotechnology.Journal of bacteriology · 2023Review
- Trimming the genomic fat: minimising and re-functionalising genomes using synthetic biology.Nature communications · 2023Review
- Refined control of CRISPR-Cas9 gene editing inFrontiers in immunology · 2023Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The availability of complete genome sequences and the definition of essential gene sets were fundamental in the start of the genome engineering era. In a recent study, redundant and unnecessary genes were systematically deleted from the Gram-positive bacterium Bacillus subtilis, an industrial production host of high-value secreted proteins. This culminated in strain PG10, which lacks about 36% of the genome, thus representing the most minimal Bacillus chassis currently available. Here, we show that this "mini Bacillus" strain has synthetic traits that are favorable for producing "difficult-to-produce proteins". As exemplified with different staphylococcal antigens, PG10 overcomes several bottlenecks in protein production related to the secretion process and instability of the secreted product. These findings show for the first time that massive genome reduction can substantially improve secretory protein production by a bacterial expression host, and underpin the high potential of genome-engineered strains as future cell factories.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.