Evidence map›Paper›PMID 30538933›Full record

ArticleOpen veterinary journal2018

Novel extraction of high quality genomic DNA from frozen bovine blood samples by using detergent method.

Talla Sridhar Goud, Ramesh Chandra Upadhyay, Anil Kumar, Satyanagalakshmi Karri, Renuka Choudhary, Syma Ashraf, Sohan Vir Singh, Onteru Suneel Kumar, Chadipiralla Kiranmai

Open access · goldAbstract read
In one paragraph

Article in Open veterinary journal, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.4field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 13 citations in OpenAlex.

  1. The FunctionalInternational journal of molecular sciences · 2026
    Article
  2. Article
  3. Article
  4. Article
  5. Review
  6. DNA Extraction with TRIzol Reagent Using a Silica Column.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2021
    Article
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Talla Sridhar GoudClimate Resilient Live Stock Research Centre, ICAR-National Dairy Research Institute, Karnal-132001, Haryana, India.
Ramesh Chandra UpadhyayClimate Resilient Live Stock Research Centre, ICAR-National Dairy Research Institute, Karnal-132001, Haryana, India.
Anil KumarClimate Resilient Live Stock Research Centre, ICAR-National Dairy Research Institute, Karnal-132001, Haryana, India.
Satyanagalakshmi KarriDepartment of Biotechnology, Vikrama Simhapuri University, Nellore-524003 Andhrapradesh, India.
Renuka ChoudharyClimate Resilient Live Stock Research Centre, ICAR-National Dairy Research Institute, Karnal-132001, Haryana, India.
Syma AshrafClimate Resilient Live Stock Research Centre, ICAR-National Dairy Research Institute, Karnal-132001, Haryana, India.
Sohan Vir SinghClimate Resilient Live Stock Research Centre, ICAR-National Dairy Research Institute, Karnal-132001, Haryana, India.
Onteru Suneel KumarMolecular Endocrinology and Structural Biology, Animal Biochemistry Division, ICAR-National Dairy Research Institute, Karnal-132001, Haryana, India.
Chadipiralla KiranmaiDepartment of Biotechnology, Vikrama Simhapuri University, Nellore-524003 Andhrapradesh, India.
National Dairy Research Institute · INVikrama Simhapuri University · IN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

DNA is the prerequisite for life's inception that transfers hereditary information, past several years; various types of commercial kits are made available which vary depending on the type of the biological sample being used. The present study is focused on developing an improvised methodology for the isolation of genomic DNA from stored bovine blood samples. DNA was isolated by using the conventional Phenol: Chloroform: Isoamyl alcohol (PCI) method and Detergent method. The aim of the study was to make a comparative analysis and evaluation of these two methods to identify the one that gives a superior quality and quantity of genomic DNA. Total (

Indexed as

DG methodgDNAMelanocortin-1-receptor genePCI methodTotal lymphocytes

Identifiers

PMID30538933
PMCPMC6243206
OpenAlexW2901263615

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-SA
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.