ArticleBiochemical and biophysical research communications2019
TGFβ2-induced tenogenesis impacts cadherin and connexin cell-cell junction proteins in mesenchymal stem cells.
Article in Biochemical and biophysical research communications, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 38 citations in OpenAlex.
- Transcriptomic and functional comparison of adipose-and bone marrow-derived mesenchymal stem cells for tendon regeneration.Stem cell research & therapy · 2026Article
- Directed Cell Self-Assembly to Form Tendon and Muscle Models for Studying Early Stages of Musculoskeletal Tissue Formation.Journal of biomechanical engineering · 2026Article
- The LINC Complex Regulates Tendon Elastic Modulus, Collagen Crimp, and Lateral Expansion During Early Postnatal Development.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2025Article
- Review
- Enthesitis on Chip - A Model for Studying Acute and Chronic Inflammation of the Enthesis and its Pharmacological Treatment.Advanced healthcare materials · 2024Article
- Recent Advances in the Use of Stem Cells in Tissue Engineering and Adjunct Therapies for Tendon Reconstruction and Future Perspectives.International journal of molecular sciences · 2024Review
- Engineering Cell-ECM-Material Interactions for Musculoskeletal Regeneration.Bioengineering (Basel, Switzerland) · 2023Review
- Stem Cell Applications and Tenogenic Differentiation Strategies for Tendon Repair.Stem cells international · 2023Review
- Growth factors in the treatment of Achilles tendon injury.Frontiers in bioengineering and biotechnology · 2023Review
- Axin2-lineage cells contribute to neonatal tendon regeneration.Connective tissue research · 2022Article
- Regulators of collagen crosslinking in developing and adult tendons.European cells & materials · 2022Review
- Nonwoven-based gelatin/polycaprolactone membrane loaded with ERK inhibitor U0126 for treatment of tendon defects.Stem cell research & therapy · 2022Article
- Maintenance of Ligament Homeostasis of Spheroid-Colonized Embroidered and Functionalized Scaffolds after 3D Stretch.International journal of molecular sciences · 2021Article
- Dielectrophoretic Characterization of Tenogenically Differentiating Mesenchymal Stem Cells.Biosensors · 2021Article
- Akt signaling is activated by TGFβ2 and impacts tenogenic induction of mesenchymal stem cells.Stem cell research & therapy · 2021Article
- Dielectrophoresis as a tool for electrophysiological characterization of stem cells.Biophysics reviews · 2020Review
- Mechanotransduction of stem cells for tendon repair.World journal of stem cells · 2020Review
- Gap junction-mediated cell-to-cell communication in oral development and oral diseases: a concise review of research progress.International journal of oral science · 2020Review
- Using tools in mechanobiology to repair tendons.Current tissue microenvironment reports · 2020Article
- Cell-cell junctions in developing and adult tendons.Tissue barriers · 2020Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Tenogenic differentiation of stem cells is needed for tendon tissue engineering approaches. A current challenge is the limited information on the cellular-level changes during tenogenic induction. Tendon cells in embryonic and adult tendons possess an array of cell-cell junction proteins that include cadherins and connexins, but how these proteins are impacted by tenogenic differentiation is unknown. Our objective was to explore how tenogenic induction of mesenchymal stem cells (MSCs) using the transforming growth factor (TGF)β2 impacted protein markers of tendon differentiation and protein levels of N-cadherin, cadherin-11 and connexin-43. MSCs were treated with TGFβ2 for 21 days. At 3 days, TGFβ2-treated MSCs developed a fibroblastic morphology and significantly decreased levels of N-cadherin protein, which were maintained through 21 days. Similar decreases in protein levels were found for cadherin-11. Connexin-43 protein levels significantly increased at 3 days, but then decreased below control levels, though not significantly. Protein levels of scleraxis and tenomodulin were significantly increased at day 14 and 21, respectively. Taken together, our results indicate that TGFβ2 is an inducer of tendon marker proteins (scleraxis and tenomodulin) in MSCs and that tenogenesis alters the protein levels of N-cadherin, cadherin-11 and connexin-43. These findings suggest a role for connexin-43 early in tenogenesis, and show that early-onset and sustained decreases in N-cadherin and cadherin-11 may be novel markers of tenogenesis in MSCs.
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Registered trials
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