ArticleMolecular microbiology2019
Periplasmic protein EipA determines envelope stress resistance and virulence in Brucella abortus.
Article in Molecular microbiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 26 citations in OpenAlex.
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- Disrupting NtrC function reveals unexpected robustness in a central cell cycle regulatory network.mBio · 2025Article
- The putative type 4 secretion system effector BspD is involved in maintaining envelope integrity of the pathogenmSphere · 2024Article
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- Cross regulation in a three-component cell envelope stress signaling system ofbioRxiv : the preprint server for biology · 2023Article
- TheAnnual review of microbiology · 2023Review
- Brucellosis detection and the role ofVeterinary world · 2023Review
- The 27th Annual Midwest Microbial Pathogenesis Conference in the Age of COVID.Journal of bacteriology · 2022Review
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- Galectin-3 regulates proinflammatory cytokine function and favours Brucella abortus chronic replication in macrophages and mice.Cellular microbiology · 2021Article
- The ChvG-ChvI and NtrY-NtrX Two-Component Systems Coordinately Regulate Growth of Caulobacter crescentus.Journal of bacteriology · 2021Article
- Brucella ovis Cysteine Biosynthesis Contributes to Peroxide Stress Survival and Fitness in the Intracellular Niche.Infection and immunity · 2021Article
- Quantification ofProceedings of the National Academy of Sciences of the United States of America · 2021Article
- The DUF1013 protein TrcR tracks with RNA polymerase to control the bacterial cell cycle and protect against antibiotics.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- β-Barrels covalently link peptidoglycan and the outer membrane in the α-proteobacterium Brucella abortus.Nature microbiology · 2021Article
- Genome Report-A Genome Sequence Analysis of the RB51 Strain ofG3 (Bethesda, Md.) · 2020Article
- A Carbonic Anhydrase Pseudogene Sensitizes SelectJournal of bacteriology · 2019Article
- Article
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12 authors at 4 institutions in 2 countries.
Funding
Abstract
Molecular components of the Brucella abortus cell envelope play a major role in its ability to infect, colonize and survive inside mammalian host cells. In this study, we have defined a role for a conserved gene of unknown function in B. abortus envelope stress resistance and infection. Expression of this gene, which we name eipA, is directly activated by the essential cell cycle regulator, CtrA. eipA encodes a soluble periplasmic protein that adopts an unusual eight-stranded β-barrel fold. Deletion of eipA attenuates replication and survival in macrophage and mouse infection models, and results in sensitivity to treatments that compromise the cell envelope integrity. Transposon disruption of genes required for LPS O-polysaccharide biosynthesis is synthetically lethal with eipA deletion. This genetic connection between O-polysaccharide and eipA is corroborated by our discovery that eipA is essential in Brucella ovis, a naturally rough species that harbors mutations in several genes required for O-polysaccharide production. Conditional depletion of eipA expression in B. ovis results in a cell chaining phenotype, providing evidence that eipA directly or indirectly influences cell division in Brucella. We conclude that EipA is a molecular determinant of Brucella virulence that functions to maintain cell envelope integrity and influences cell division.
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