Evidence map›Paper›PMID 30536579›Full record

ArticleCytometry. Part A : the journal of the International Society for Analytical Cytology2019

Rapid Assessment of Mitochondrial Complex I Activity and Metabolic Phenotyping of Breast Cancer Cells by NAD(p)H Cytometry.

V Krishnan Ramanujan

Abstract read
In one paragraph

Article in Cytometry. Part A : the journal of the International Society for Analytical Cytology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

1 author.

V Krishnan RamanujanMetabolic Photonics Laboratory, Departments of Surgery and Biomedical Sciences, Cedars-Sinai Medical Center, 8700 Beverly Blvd, Los Angeles, California, 90048.

Funding

Multiphoton Fluorescence Lifetime Imaging of Solid Tumors InvivoR21CA124843 · NCI · CEDARS-SINAI MEDICAL CENTER · PI RAMANUJAN, V.KRISHNAN · 2009 to 2010
$378k
NCI NIH HHS R21 CA124843
6 · The paper itself

Abstract

Cancer cells are known to display a variety of metabolic reprogramming strategies to fulfill their own growth and proliferative agenda. With the advent of high resolution imaging strategies, metabolomics techniques, and so forth, there is an increasing appreciation of critical role that tumor cell metabolism plays in the overall breast cancer (BC) growth. In this report, we demonstrate a sensitive, flow-cytometry-based assay for rapidly assessing the metabolic phenotypes in isolated suspensions of breast cancer cells. By measuring the temporal variation of NAD(p)H signals in unlabeled, living cancer cells, and by measuring mitochondrial membrane potential {Δψ

Indexed as

AnimalsBreast NeoplasmsCell PlasticityDisease Models, AnimalElectron Transport Complex IFemaleFlow CytometryHumansMCF-7 CellsMiceMitochondriaNADSingle-Cell AnalysisElectron Transport Complex INADbreast cancermetabolic plasticitymetabolismmitochondriaNAD(p)Htumor heterogeneity

Identifiers

PMID30536579
PMCPMC6329654

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.