ArticleThe Plant journal : for cell and molecular biology2019
Identification, characterization and field testing of Brassica napus mutants producing high-oleic oils.
Article in The Plant journal : for cell and molecular biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 39 citations in OpenAlex.
- Multiplex FAD2/FATB Editing Generates Ultra-High-Oleic, Low-Saturate Soybean With Increased Seed Fatty Acid Content.Plant biotechnology journal · 2026Article
- Towards premium rapeseed oil: leveraging allelic variation in BnaFAD2.A5, BnaFAD3.A4 and BnaFAD3.C4 for molecular design breeding.BMC plant biology · 2026Article
- Genetic Regulation and Breeding Application of Medium-Chain Fatty Acids Metabolism in Rice.Biology · 2025Review
- KeyInternational journal of molecular sciences · 2023Article
- The coexpression of two desaturases provides an optimized reduction of saturates in camelina oil.Plant biotechnology journal · 2023Article
- Bioengineering of Soybean Oil and Its Impact on Agronomic Traits.International journal of molecular sciences · 2023Review
- CRISPR/Cas9-Mediated Gene Editing ofGenes · 2022Article
- Molecular Approaches Reduce Saturates and EliminateFrontiers in plant science · 2022Review
- Allelic Variation inFrontiers in nutrition · 2022Article
- Concerted increases ofPlant direct · 2021Article
- Identification and Development of KASP Markers for Novel MutantFrontiers in plant science · 2021Article
- Cleaved amplified polymorphic sequences (CAPS) marker for identification of two mutant alleles of the rapeseed BnaA.FAD2 gene.Molecular biology reports · 2020Article
- Modifications of fatty acid profile through targeted mutation at BnaFAD2 gene with CRISPR/Cas9-mediated gene editing in Brassica napus.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2020Article
- The biochemistry of headgroup exchange during triacylglycerol synthesis in canola.The Plant journal : for cell and molecular biology · 2020Article
- Characterization and validation of olive FAD and SAD gene families: expression analysis in different tissues and during fruit development.Molecular biology reports · 2020Article
- The impact of reducing fatty acid desaturation on the composition and thermal stability of rapeseed oil.Plant biotechnology journal · 2020Article
- A novel quantitative trait locus on chromosome A9 controlling oleic acid content in Brassica napus.Plant biotechnology journal · 2019Article
- Engineering the stereoisomeric structure of seed oil to mimic human milk fat.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
Abstract
Producing healthy, high-oleic oils and eliminating trans-fatty acids from foods are two goals that can be addressed by reducing activity of the oleate desaturase, FAD2, in oilseeds. However, it is essential to understand the consequences of reducing FAD2 activity on the metabolism, cell biology and physiology of oilseed crop plants. Here, we translate knowledge from studies of fad2 mutants in Arabidopsis (Arabidopsis thaliana) to investigate the limits of non-GMO approaches to maximize oleic acid in the seed oil of canola (Brassica napus), a species that expresses three active FAD2 isozymes. A series of hypomorphic and null mutations in the FAD2.A5 isoform were characterized in yeast (Saccharomyes cerevisiae). Then, four of these were combined with null mutations in the other two isozymes, FAD2.C5 and FAD2.C1. The resulting mutant lines contained 71-87% oleic acid in their seed oil, compared with 62% in wild-type controls. All the mutant lines grew well in a greenhouse, but in field experiments we observed a clear demarcation in plant performance. Mutant lines containing less than 80% oleate in the seed oil were indistinguishable from wild-type controls in growth parameters and seed oil content. By contrast, lines with more than 80% oleate in the seed oil had significantly lower seedling establishment and vigor, delayed flowering and reduced plant height at maturity. These lines also had 7-11% reductions in seed oil content. Our results extend understanding of the B. napusFAD2 isozymes and define the practical limit to increasing oil oleate content in this crop species.
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