Evidence map›Paper›PMID 30536192›Full record

ArticleInternational urology and nephrology2019

Increased levels of serum pigment epithelium-derived factor aggravate proteinuria via induction of podocyte actin rearrangement.

Na Huang, Xuan Zhang, Youzhao Jiang, Hao Mei, Ling Zhang, Qiong Zhang, Jiongyu Hu, Bing Chen

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International urology and nephrology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.1field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 12 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 5 institutions in 2 countries.

Na HuangDepartment of Endocrinology, Southwest Hospital, Third Military Medical University (Army Medical University), 29 Gaotanyan Street, Chongqing, 400038, China.
Xuan ZhangDepartment of Neurosurgery, Southwest Hospital, Third Military Medical University (Army Medical University), 29 Gaotanyan Street, Chongqing, 400038, China.
Youzhao JiangDepartment of Endocrinology, Banan People's Hospital of Chongqing, 2 Xinnong Street, Chongqing, 401320, China.
Hao MeiCenter for Outcomes Research and Evaluation, Yale-New Haven Hospital, New Haven, CT, 06510, USA.
Ling ZhangOutpatient Department, Southwest Hospital, Third Military Medical University (Army Medical University), 29 Gaotanyan Street, Chongqing, 400038, China.
Qiong ZhangInstitute of Burn Research, State Key Laboratory of Trauma, Burns and Combined Injury, Southwest Hospital, Third Military Medical University (Army Medical University), 29 Gaotanyan Street, Chongqing, 400038, China.
Jiongyu HuDepartment of Endocrinology, State Key Laboratory of Trauma, Burns and Combined Injury, Southwest Hospital, Third Military Medical University (Army Medical University), 29 Gaotanyan Street, Chongqing, 400038, China.
Bing ChenDepartment of Endocrinology, Southwest Hospital, Third Military Medical University (Army Medical University), 29 Gaotanyan Street, Chongqing, 400038, China. xnyychenbing@163.com.
Southwest Hospital · CNArmy Medical University · CNFirst People's Hospital of Chongqing · CNLuoyang Central Hospital Affiliated to Zhengzhou University · CNYale New Haven Hospital · US

Funding

grant support from the Southwest Hospital SWH2016JCYB-06National Natural Science Foundation of China 81570731
6 · The paper itself

Abstract

purposeTo assess the role of serum pigment epithelium-derived factor (PEDF) in the occurrence and development of proteinuria and renal dysfunction and determine its relevant signaling pathway.

methodsWe analyzed serum PEDF, creatinine, the urinary albumin-to-creatinine ratio, and renal morphology of normal or streptozotocin (STZ)-induced diabetic mice, before and after treatment with PEDF. In vitro, podocytes were stimulated with PEDF under normal or high-glucose conditions; permeability was measured by the transwell assay with fluorescein isothiocyanate (FITC)-dextran; and F-actin cytoskeleton was analyzed by phalloidin staining. Apoptosis was assessed by flow cytometry. RhoA activity and ROCK1, ZO-1, nephrin, and podocin levels were detected by Western blotting.

resultsDiabetic mice exhibited a high serum PEDF level. In vivo, elevated serum PEDF led to proteinuria, increased serum creatinine, and podocyte foot process fusion in normal or diabetic mice. In vitro, both high-glucose and PEDF stimulation activated the RhoA/ROCK1 pathway in podocytes and promoted cell permeability, F-actin rearrangement, and apoptosis. Inhibition of RhoA/ROCK1 alleviated the damage from these effects.

conclusionsElevated serum PEDF aggravates the development of proteinuria and renal dysfunction by inducing F-actin arrangement, foot process fusion, and apoptosis of podocytes in both normal and diabetic mice, and this effect may be mediated by activation of the RhoA/ROCK1 pathway.

Indexed as

KidneyProteinuriaActinsAnimalsApoptosisDiabetes Mellitus, ExperimentalDiabetic NephropathiesEye ProteinsKidney Function TestsMiceNerve Growth FactorsPigment Epithelium-Derived FactorPodocytesrhoA GTP-Binding Proteinrho-Associated Kinasesrho GTP-Binding ProteinsActinsEye ProteinsNerve Growth FactorsPigment Epithelium-Derived FactorrhoA GTP-Binding ProteinRhoA protein, mouserho-Associated Kinasesrho GTP-Binding ProteinsRock1 protein, mouseSerpinsActinDiabetic kidney diseasePEDFProteinuriaRhoA/ROCK1

Identifiers

PMID30536192
PMCPMC6394770
OpenAlexW2904009409

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.