ArticleParasitology research2019
A new one-step multiplex PCR assay for simultaneous detection and identification of avian haemosporidian parasites.
Article in Parasitology research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed.
- Review of Genetic Information of Morphologically Described Haemoproteus Parasites Recorded in GenBank and MalAvi Databases.Integrative zoology · 2026Review
- Heterogeneous distribution of avian malaria parasites across mosquito species: disproportionate involvement of Culex restuans in the Midwestern United States.Malaria journal · 2026Article
- Avian haemosporidian parasites in the African houbara: Species diversity and impact on blood parameters.Parasitology research · 2026Article
- Molecular prevalence and phylogenetic characterization ofVeterinary world · 2026Article
- Unveiling Hidden Threats: Molecular Detection and Genetic Diversity of Hepatozoon spp. and Toxoplasma Gondii in Wild Rodents of Saudi Arabia and Their Ectoparasites.Acta parasitologica · 2026Article
- Blood Parasites and Wildlife: The Development of a Discipline.Integrative zoology · 2026Review
- Within- and between-brood variations in haemosporidian infections of nestlings reveal complex host-vector-parasite relationships.Journal of ornithology · 2026Article
- Prevalence and Genetic Diversity of Haemoproteus and Leucocytozoon in Raptors and Other Captive Birds at the National Zoological Garden in South Africa.Integrative zoology · 2026Article
- Use of Contaminated Habitat and Associated Selenium Uptake Mediate Haemosporidian Parasite Infections in Wild Passerine Birds.Ecology and evolution · 2026Article
- Simultaneous population genomics of hosts and their parasites with selective whole genome amplification.Parasites & vectors · 2025Article
- Combined Evidence Reveals the Origin of a Rapid Range Expansion Despite Retained Genetic Diversity and a Weak Founder Effect.Molecular ecology · 2025Article
- Haemosporidian Infection Risk Variation Across an Urban Gradient in a Songbird.Ecology and evolution · 2025Article
- Avian haemosporidian parasite prevalence and diversity in two populations of the American kestrel (Falco sparverius).Parasitology research · 2025Article
- Avian Haemosporidian Parasites in Three Wild Columbids from Germany.Microorganisms · 2025Article
- Leading report on the molecular prevalence of emerging pathogens Hepatozoon sp. and Lankesterella sp. in the blood samples of seven wild lizard species.Scientific reports · 2025Article
- Characterization of co-infections of haemosporidian parasites in SwinhoeCurrent research in parasitology & vector-borne diseases · 2025Article
- MultiplexPeerJ · 2025Article
- The prevalence and immune response to coinfection by avian haemosporidians in wild Eurasian blackbirdsParasitology · 2024Article
- Avian malaria in a feral-pet pigeon: a case report.Malaria journal · 2024Article
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5 authors.
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Abstract
Accurate detection and identification are essential components for epidemiological, ecological, and evolutionary surveys of avian haemosporidian parasites. Microscopy has been used for more than 100 years to detect and identify these parasites; however, this technique requires considerable training and high-level expertise. Several PCR methods with highly sensitive and specific detection capabilities have now been developed in addition to microscopic examination. However, recent studies have shown that these molecular protocols are insufficient at detecting mixed infections of different haemosporidian parasite species and genetic lineages. In this study, we developed a simple, sensitive, and specific multiplex PCR assay for simultaneous detection and discrimination of parasites of the genera Plasmodium, Haemoproteus, and Leucocytozoon in single and mixed infections. Relative quantification of parasite DNA using qPCR showed that the multiplex PCR can amplify parasite DNA ranging in concentration over several orders of magnitude. The detection specificity and sensitivity of this new multiplex PCR assay were also tested in two different laboratories using previously screened natural single and mixed infections. These findings show that the multiplex PCR designed here is highly effective at identifying both single and mixed infections from all three genera of avian haemosporidian parasites. We predict that this one-step multiplex PCR assay, being convenient and inexpensive, will become a widely used method for molecular screening of avian haemosporidian parasites.
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