Evidence map›Paper›PMID 30536009›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2019

BCL-2 Protein Family Interaction Analysis by Nuclear Magnetic Resonance Spectroscopy.

Thomas P Garner, Evripidis Gavathiotis

Abstract read
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Thomas P GarnerDepartment of Biochemistry, Albert Einstein College of Medicine, Bronx, NY, USA.
Evripidis GavathiotisDepartment of Biochemistry, Albert Einstein College of Medicine, Bronx, NY, USA. evripidis.gavathiotis@einstein.yu.edu.

Funding

WORD PROCESSORP30CA013330 · NCI · YESHIVA UNIVERSITY · PI STEVEN C. ALMO · 1985 to 2026
$111.2M
Small Molecule Activators of Pro-apoptotic BAX for Cancer TherapyR01CA178394 · NCI · ALBERT EINSTEIN COLLEGE OF MEDICINE, INC · PI GAVATHIOTIS, EVRIPIDIS · 2014 to 2024
$3.7M
Console and probe upgrade for 600 MHz NMRS10OD016305 · OD · ALBERT EINSTEIN COLLEGE OF MEDICINE · PI COWBURN, DAVID · 2014 to 2014
$595k
NCI NIH HHS P30 CA013330NCI NIH HHS R01 CA178394NIH HHS S10 OD016305
6 · The paper itself

Abstract

Biomolecular nuclear magnetic resonance (NMR) is a powerful and versatile method for studying both protein-protein interactions (PPIs) and protein-small molecule binding. NMR has been used extensively in the investigation of BCL-2 family proteins revealing the structure of key family members, identifying binding partners and interaction sites, and screening small molecule modulators. In this chapter we discuss the application of NMR to identify interaction sites and structure determination of protein-protein and protein-small molecule complexes using two examples.

Indexed as

Binding SitesCell LineHumansNuclear Magnetic Resonance, BiomolecularProtein BindingProtein Interaction Domains and MotifsProto-Oncogene Proteins c-bcl-2Small Molecule LibrariesProto-Oncogene Proteins c-bcl-2Small Molecule LibrariesBAXBCL-2BCL-2 familyChemical shift perturbation (CSP)MCL-1Nuclear magnetic resonance (NMR)Paramagnetic relaxation enhancement (PRE)Protein–protein interactions (PPI)

Identifiers

PMID30536009
PMCPMC6508860

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.