Evidence map›Paper›PMID 30536003›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2019

Photocrosslinking Approach to Investigate Protein Interactions in the BCL-2 Family.

Jialing Lin, Arthur E Johnson, Zhi Zhang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed, 2 pooled it
10.7field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 2 syntheses or guidelines pooled it, 7 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Jialing LinDepartment of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA. jialing-lin@ouhsc.edu.
Arthur E JohnsonReCode Therapeutics, Inc., Dallas, TX, USA.
Zhi ZhangDepartment of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA.
University of Oklahoma Health Sciences Center · US

Funding

Structure-Function Studies of MsvR, a Methanogen-Specific Transcriptional RegulaP20GM103640 · NIGMS · UNIVERSITY OF OKLAHOMA · PI THOMAS, LEONARD M · 2012 to 2021
$20.4M
Structure-Function of Bcl-2 Related Apoptosis Regulators in MembranesR01GM062964 · NIGMS · UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR · PI LIN, JIALING · 2001 to 2014
$3.3M
NIGMS NIH HHS P20 GM103640NIGMS NIH HHS R01 GM062964
6 · The paper itself

Abstract

The Bcl-2 family of proteins regulates mitochondrial outer membrane permeability thereby making life or death decisions for cells. Most of Bcl-2 proteins contain hydrophobic regions that are embedded in intracellular membranes such as mitochondria. These membrane proteins are difficult to express and purify thereby preluding biochemical and biophysical characterizations. Here, we describe a photocrosslinking approach based on in vitro synthesis of Bcl-2 proteins with photoreactive amino acid analogs incorporated at specific locations. These photoreactive proteins are reconstituted into liposomal membranes with defined phospholipids or mitochondrial membranes isolated from animals, and their interactions with other Bcl-2 proteins are detected by photocrosslinking.

Indexed as

Amino AcidsAnimalsHumansLiposomesMembrane ProteinsMiceMitochondriaMitochondrial MembranesPermeabilityPhospholipidsProtein Interaction Domains and MotifsProto-Oncogene Proteins c-bcl-2Amino AcidsLiposomesMembrane ProteinsPhospholipidsProto-Oncogene Proteins c-bcl-2ApoptosisBaxBcl-2BH3-only proteinsMembrane permeabilizationMembrane protein interactionMitochondriaPhotocrosslinking

Identifiers

PMID30536003
PMCPMC6467084
OpenAlexW2904669430

What OpenQuestion holds

Textmetadata
LicenceTDM
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.