ArticleJournal of industrial microbiology & biotechnology2019
Modification of isoprene synthesis to enable production of curcurbitadienol synthesis in Saccharomyces cerevisiae.
Article in Journal of industrial microbiology & biotechnology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed, 22 citations in OpenAlex.
- A review ofChinese herbal medicines · 2026Review
- Biosynthesis and metabolic engineering of natural sweeteners.AMB Express · 2025Review
- Selection of Reference Genes inPlants (Basel, Switzerland) · 2024Article
- Biosynthetic pathway of prescription cucurbitacin IIa and high-level production of key triterpenoid intermediates in engineered yeast and tobacco.Plant communications · 2024Article
- Post-Ripening and Key Glycosyltransferase Catalysis to Promote Sweet Mogrosides Accumulation ofMolecules (Basel, Switzerland) · 2023Article
- Genetic regulation and fermentation strategy for squalene production in Schizochytrium sp.Applied microbiology and biotechnology · 2022Article
- Construction and Optimization of theFrontiers in bioengineering and biotechnology · 2022Article
- Biosynthesis of a novel ganoderic acid by expressing CYP genes from Ganoderma lucidum in Saccharomyces cerevisiae.Applied microbiology and biotechnology · 2022Article
- A review on the phytochemistry and pharmacology of the herbRSC advances · 2021Review
- Production of bioactive plant secondary metabolites through in vitro technologies-status and outlook.Applied microbiology and biotechnology · 2021Review
- Combined Biosynthetic Pathway Engineering and Storage Pool Expansion for High-Level Production of Ergosterol in IndustrialFrontiers in bioengineering and biotechnology · 2021Article
- Improved Production and In Situ Recovery of Sesquiterpene (+)-Zizaene from Metabolically-EngineeredMolecules (Basel, Switzerland) · 2019Article
- Identification of a Novel Specific Cucurbitadienol Synthase Allele inMolecules (Basel, Switzerland) · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
Cucurbitane-type triterpenoids such as mogrosides and cucurbitacins that are present in the plants of Cucurbitaceae are widely used in Asian traditional medicine. Cucurbitadienol is the skeleton of cucurbitane-type triterpenoids. As an alternative production strategy, we developed baker's yeast Saccharomyces cerevisiae as a microbial host for the eventual transformation of cucurbitadienol. The synthetic pathway of cucurbitadienol was constructed in Saccharomyces cerevisiae by introducing the cucurbitadienol synthase gene from different plants, resulting in 7.80 mg cucurbitadienol from 1 L of fermentation broth. Improving supplies of isoprenoid precursors was then investigated for increasing cucurbitadienol production. Cucurbitadienol production increased to 21.47 mg/L through the overexpression of a global regulatory factor (UPC2) gene of triterpenoid synthase. In addition, knockout of the ERG7 gene increased cucurbitadienol production from 21.47 to 61.80 mg/L. Finally, fed-batch fermentation was performed, and 63.00 mg/L cucurbitadienol was produced. This work is an important step towards the total biosynthesis of valuable cucurbitane-type triterpenoids and demonstrates the potential for developing a sustainable and secure yeast biomanufacturing platform for triterpenoids.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.