ArticleJournal of experimental botany2019
The mitochondrial pentatricopeptide repeat protein EMP12 is involved in the splicing of three nad2 introns and seed development in maize.
Article in Journal of experimental botany, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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30 citing papers in PubMed, 55 citations in OpenAlex.
- Pentatricopeptide repeat proteins in crops: Advances in functional mechanisms and breeding applications.Journal of integrative plant biology · 2026Review
- Article
- Review
- A translatome-transcriptome multi-omics gene regulatory network reveals the complicated functional landscape of maize.Genome biology · 2023Article
- A Systemic Investigation of Genetic Architecture and Gene Resources Controlling Kernel Size-Related Traits in Maize.International journal of molecular sciences · 2023Article
- Identification of a novel locusFrontiers in genetics · 2023Article
- Pentatricopeptide repeat protein CNS1 regulates maize mitochondrial complex III assembly and seed development.Plant physiology · 2022Article
- Maize PPR278 Functions in Mitochondrial RNA Splicing and Editing.International journal of molecular sciences · 2022Article
- Genetic Architecture of Grain Yield-Related Traits in Sorghum and Maize.International journal of molecular sciences · 2022Review
- ZmnMAT1, a nuclear-encoded type I maturase, is required for the splicing of mitochondrialFrontiers in plant science · 2022Article
- OsNBL3, a mitochondrion-localized pentatricopeptide repeat protein, is involved in splicing nad5 intron 4 and its disruption causes lesion mimic phenotype with enhanced resistance to biotic and abiotic stresses.Plant biotechnology journal · 2021Article
- Functions of PPR Proteins in Plant Growth and Development.International journal of molecular sciences · 2021Review
- Rice FLOURY SHRUNKEN ENDOSPERM 5 Encodes a Putative Plant Organelle RNA Recognition Protein that Is Required for cis-Splicing of Mitochondrial nad4 Intron 1.Rice (New York, N.Y.) · 2021Article
- Cotton Fiber Development Requires the Pentatricopeptide Repeat Protein GhIm for Splicing of Mitochondrial nad7 mRNA.Genetics · 2021Article
- OsPPR939, a nad5 splicing factor, is essential for plant growth and pollen development in rice.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2021Article
- Maize kernel development.Molecular breeding : new strategies in plant improvement · 2021Review
- Regulator of Chromosome Condensation 1-Domain Protein DEK47 Functions on the Intron Splicing of MitochondrialFrontiers in plant science · 2021Article
- Roles of Reactive Oxygen Species and Mitochondria in Seed Germination.Frontiers in plant science · 2021Review
- A Genome-Wide Analysis of theGenes · 2020Article
- The Kernel Size-Related Quantitative Trait LocusPlant physiology · 2020Article
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10 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Plant mitochondrial genes contain cis- and trans-group II introns that must be spliced before translation. The mechanism by which these introns are spliced is not well understood. Several families of proteins have been implicated in the intron splicing, of which the pentatricopeptide repeat (PPR) proteins are proposed to confer the substrate binding specificity. However, very few PPRs are characterized. Here, we report the function of a P-type PPR protein, EMP12, and its role in seed development. EMP12 is targeted to mitochondria. Loss-of-function mutation in Emp12 severely arrests embryo and endosperm development, causing embryo lethality. The trans-splicing of mitochondrial nad2 intron 2 and cis-splicing of nad2 intron 4 are abolished, whereas the cis-splicing of nad2 intron 1 is reduced in emp12 mutants. As a result, complex I assembly is disrupted, and its activity is strongly reduced in the mutants. The expression of the alternative oxidase and several components of other mitochondrial complexes is increased, possibly in response to the defective complex I. These results suggest that Emp12 is required for the trans-splicing of nad2 intron 2 and cis-splicing of nad2 introns 1 and 4, and is important to complex I biogenesis, and embryogenesis and endosperm development in maize.
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