ArticleAnnals of botany2019
Nitrate nutrition influences multiple factors in order to increase energy efficiency under hypoxia in Arabidopsis.
Article in Annals of botany, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 40 citations in OpenAlex.
- Physiological Responses of Apple to Nitrogen Fertilization Regimes: Roles of Calcium Metabolism in Fruit Quality and Bitter Pit Development.Plants (Basel, Switzerland) · 2026Article
- The role of alternative oxidase in the maintenance of cellular redox balance under hypoxia via participation in nitric oxide turnover.Journal of experimental botany · 2025Review
- Strong scale-dependent relationships between fine-root function and soil properties uncovered with spatially coupled sampling.The New phytologist · 2025Article
- Nitrogen reduces calcium availability by promoting oxalate biosynthesis in apple leaves.Horticulture research · 2024Article
- Article
- Elucidating the molecular responses to waterlogging stress in onion (Frontiers in plant science · 2023Article
- Nitrate-Nitrite-Nitric Oxide Pathway: A Mechanism of Hypoxia and Anoxia Tolerance in Plants.International journal of molecular sciences · 2022Review
- Nitrate alleviates ammonium toxicity in wheat (Plant signaling & behavior · 2021Article
- Roles for Plant Mitochondrial Alternative Oxidase Under Normoxia, Hypoxia, and Reoxygenation Conditions.Frontiers in plant science · 2020Article
- Potential Pathway of Nitrous Oxide Formation in Plants.Frontiers in plant science · 2020Review
- Current approaches to measure nitric oxide in plants.Journal of experimental botany · 2019Review
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Authors and funding
10 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND AND
aimsNitrogen (N) levels vary between ecosystems, while the form of available N has a substantial impact on growth, development and perception of stress. Plants have the capacity to assimilate N in the form of either nitrate (NO3-) or ammonium (NH4+). Recent studies revealed that NO3- nutrition increases nitric oxide (NO) levels under hypoxia. When oxygen availability changes, plants need to generate energy to protect themselves against hypoxia-induced damage. As the effects of NO3- or NH4+ nutrition on energy production remain unresolved, this study was conducted to investigate the role of N source on group VII transcription factors, fermentative genes, energy metabolism and respiration under normoxic and hypoxic conditions.
methodsWe used Arabidopsis plants grown on Hoagland medium with either NO3- or NH4+ as a source of N and exposed to 0.8 % oxygen environment. In both roots and seedlings, we investigated the phytoglobin-nitric oxide cycle and the pathways of fermentation and respiration; furthermore, NO levels were tested using a combination of techniques including diaminofluorescein fluorescence, the gas phase Griess reagent assay, respiration by using an oxygen sensor and gene expression analysis by real-time quantitative reverse transcription-PCR methods. KEY
resultsUnder NO3- nutrition, hypoxic stress leads to increases in nitrate reductase activity, NO production, class 1 phytoglobin transcript abundance and metphytoglobin reductase activity. In contrast, none of these processes responded to hypoxia under NH4+ nutrition. Under NO3- nutrition, a decreased total respiratory rate and increased alternative oxidase capacity and expression were observed during hypoxia. Data correlated with decreased reactive oxygen species and lipid peroxidation levels. Moreover, increased fermentation and NAD+ recycling as well as increased ATP production concomitant with the increased expression of transcription factor genes HRE1, HRE2, RAP2.2 and RAP2.12 were observed during hypoxia under NO3- nutrition.
conclusionsThe results of this study collectively indicate that nitrate nutrition influences multiple factors in order to increase energy efficiency under hypoxia.
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