ArticleThe Journal of infectious diseases2019
In Silico and Experimental Evaluation of Primer Sets for Species-Level Resolution of the Vaginal Microbiota Using 16S Ribosomal RNA Gene Sequencing.
Article in The Journal of infectious diseases, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT02272231 (Pathogenesis of Bacterial Vaginosis in Women Who Have Sex With Women), which is not on this map. Cited by 35 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Pathogenesis of Bacterial Vaginosis in Women Who Have Sex With Women
Who cites it
35 citing papers in PubMed, 47 citations in OpenAlex.
- A Randomized Controlled Crossover Lifestyle Intervention to Improve Metabolic and Mental Health in Female Healthcare Night-Shift Workers.Nutrients · 2025Trial
- Vegetable Gardening and Health Outcomes in Older Cancer Survivors: A Randomized Clinical Trial.JAMA network open · 2024Trial
- Cervicovaginal microbiome and natural history of HPV in a longitudinal study.PLoS pathogens · 2020Trial
- Sexual practices have a significant impact on the vaginal microbiota of women who have sex with women.Scientific reports · 2019Trial
- Predicting bacterial vaginosis incidence using artificial neural networks.PLoS computational biology · 2026Article
- Complement and neutrophils are actively involved at the cervicovaginal interface in cases of adverse microbial composition, cervical shortening and spontaneous preterm birth.NPJ biofilms and microbiomes · 2026Article
- Beyond community state types: vaginal microbial diversity and CST-IV signatures are associated with pregnancy in euploid embryo transfer cycles.Frontiers in endocrinology · 2026Article
- Epigenetic and microbiome responses to greens supplementation in obese older adults: results from a randomized crossover-controlled trial.Frontiers in nutrition · 2026Article
- Methods to characterize the vaginal microbiome in a rhesus macaque model of simian human immunodeficiency virus (SHIV) transmission uncover epithelium-associated enrichment ofCurrent research in microbial sciences · 2026Article
- Functional mouth rinse containing inorganic nitrate and antioxidants bolsters the enterosalivary pathway and lowers blood pressure in Wistar rats.Scientific reports · 2025Article
- The MR1/MAIT cell axis impacts the gut-brain axis through both cognition and microbial community structure in 5XFAD mice.Alzheimer's & dementia : the journal of the Alzheimer's Association · 2025Article
- Article
- Fecal Microbiota and Associated Metabolites Are Minimally Affected by Ten Weeks of Resistance Training in Younger and Older Adults.Sports (Basel, Switzerland) · 2025Article
- Article
- Time-restricted feeding mediated modulation of microbiota leads to changes in muscle physiology in Drosophila obesity models.Aging cell · 2025Article
- A Narrative Review of the Putative Etiologic Role and Diagnostic Utility of the Cervicovaginal Microbiome in Human Papillomavirus-Associated Cervical Carcinogenesis.Journal of medical virology · 2024Review
- Article
- Microbial interactions amongBMJ open · 2024Article
- Group BFrontiers in cellular and infection microbiology · 2024Article
- Change in microbiota profile after vaginal estriol cream in postmenopausal women with stress incontinence.Frontiers in microbiology · 2024Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
Background: Identification of bacteria in human vaginal specimens is commonly performed using 16S ribosomal RNA (rRNA) gene sequences. However, studies utilize different 16S primer sets, sequence databases, and parameters for sample and database clustering. Our goal was to assess the ability of these methods to detect common species of vaginal bacteria. Methods: We performed an in silico analysis of 16S rRNA gene primer sets, targeting different hypervariable regions. Using vaginal samples from women with bacterial vaginosis, we sequenced 16S genes using the V1-V3, V3-V4, and V4 primer sets. For analysis, we used an extended Greengenes database including 16S gene sequences from vaginal bacteria not already present. We compared results with those obtained using the SILVA 16S database. Using multiple database and sample clustering parameters, each primer set's ability to detect common vaginal bacteria at the species level was determined. We also compared these methods to the use of DADA2 for denoising and clustering of sequence reads. Results: V4 sequence reads clustered at 99% identity and using the 99% clustered, extended Greengenes database provided optimal species-level identification of vaginal bacteria. Conclusions: This study is a first step toward standardizing methods for 16S rRNA gene sequencing and bioinformatics analysis of vaginal microbiome data.
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Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.