ArticleLab on a chip2019
Microfluidic long DNA sample preparation from cells.
Article in Lab on a chip, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
6 citing papers in PubMed.
- Electroelution Into a Salt Trap: Reviving an Old-School Approach to DNA Purification.Small methods · 2026Article
- Microfluidic purification of genomic DNA.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Microfluidic-based electrically driven particle manipulation techniques for biomedical applications.RSC advances · 2025Review
- Recent advancements in nucleic acid detection with microfluidic chip for molecular diagnostics.Trends in analytical chemistry : TRAC · 2023Review
- 3D Printing-Enabled DNA Extraction for Long-Read Genomics.ACS omega · 2020Article
- A bifurcated continuous field-flow fractionation (BCFFF) chip for high-yield and high-throughput nucleic acid extraction and purification.Lab on a chip · 2019Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
A number of outstanding problems in genomics, such as identifying structural variations and sequencing through centromeres and telomeres, stand poised to benefit tremendously from emerging long-read genomics technologies such as nanopore sequencing and genome mapping in nanochannels. However, optimal application of these new genomics technologies requires facile methods for extracting long DNA from cells. These sample preparation tools should be amenable to automation and minimize fragmentation of the long DNA molecules by shear. We present one such approach in a poly(dimethylsiloxane) device, where gel-based high molecular weight DNA extraction and continuous flow purification in a 3D cell culture-inspired geometry is followed by electrophoretic extraction of the long DNA from the miniaturized gel. Molecular combing reveals that the device produces molecules that are typically in excess of 100 kilobase pairs in size, with the longest molecule extending up to 4 megabase pairs. The microfluidic format reduces the standard day-long and labor-intensive DNA extraction process to 4 hours, making it a promising prototype platform for routine long DNA sample preparation.
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Registered trials
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