ArticleCommunications biology2018
Rapid characterization of secreted recombinant proteins by native mass spectrometry.
Article in Communications biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The trial behind it
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Who cites it
16 citing papers in PubMed, 33 citations in OpenAlex.
- Differences in α-synuclein conformational states in physiologically relevant pH/NaThe Analyst · 2026Article
- Epitope Mapping of Japanese Encephalitis Virus Neutralizing Antibodies by Native Mass Spectrometry and Hydrogen/Deuterium Exchange.Biomolecules · 2024Article
- The Complementarity of Nuclear Magnetic Resonance and Native Mass Spectrometry in Probing Protein-Protein Interactions.Advances in experimental medicine and biology · 2024Review
- Native Mass Spectrometry: Recent Progress and Remaining Challenges.Annual review of biophysics · 2022Review
- Approaches to Heterogeneity in Native Mass Spectrometry.Chemical reviews · 2022Review
- Surface-Induced Dissociation for Protein Complex Characterization.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Direct-MS analysis of antibody-antigen complexes.Proteomics · 2021Article
- Intracellular Protein-Drug Interactions Probed by Direct Mass Spectrometry of Cell Lysates.Angewandte Chemie (International ed. in English) · 2021Article
- Analysis of Tagged Proteins Using Tandem Affinity-Buffer Exchange Chromatography Online with Native Mass Spectrometry.Biochemistry · 2021Article
- Carbohydrate binding module-fused antibodies improve the performance of cellulose-based lateral flow immunoassays.Scientific reports · 2021Article
- Use of tandem affinity-buffer exchange chromatography online with native mass spectrometry for optimizing overexpression and purification of recombinant proteins.Methods in enzymology · 2021Article
- Mass Spectrometry Analysis of Intact Proteins from Crude Samples.Analytical chemistry · 2020Article
- Rapid online buffer exchange for screening of proteins, protein complexes and cell lysates by native mass spectrometry.Nature protocols · 2020Article
- Direct characterization of overproduced proteins by native mass spectrometry.Nature protocols · 2020Article
- Improvement of solubility and yield of recombinant protein expression inResearch in pharmaceutical sciences · 2019Article
- Optimizing antibody affinity and stability by the automated design of the variable light-heavy chain interfaces.PLoS computational biology · 2019Article
Corrections and comments
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Authors and funding
12 authors at 1 institution in 1 country.
Funding
Abstract
Characterization of overexpressed proteins is essential for assessing their quality, and providing input for iterative redesign and optimization. This process is typically carried out following purification procedures that require pronounced cost of time and labor. Therefore, quality assessment of recombinant proteins with no prior purification offers a major advantage. Here, we report a native mass spectrometry method that enables characterization of overproduced proteins directly from culture media. Properties such as solubility, molecular weight, folding, assembly state, overall structure, post-translational modifications and binding to relevant biomolecules are immediately revealed. We show the applicability of the method for in-depth characterization of secreted recombinant proteins from eukaryotic systems such as yeast, insect, and human cells. This method, which can be readily extended to high-throughput analysis, considerably shortens the time gap between protein production and characterization, and is particularly suitable for characterizing engineered and mutated proteins, and optimizing yield and quality of overexpressed proteins.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.