Evidence map›Paper›PMID 30534591›Full record

ArticleTransplantation direct2018

A Comparative Study of Single and Dual Perfusion During End-ischemic Subnormothermic Liver Machine Preservation.

Isabel M A Brüggenwirth, Carolina Moore, Paria Mahboub, Max F Thijssen, Xiaofei E, Henri G D Leuvenink, Pranoti Mandrekar, Xiaofei Wang, Timothy F Kowalik, Robert J Porte and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Transplantation direct, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.7field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 10 citations in OpenAlex.

  1. A compact machine perfusion device for whole blood perfusion in isolated rat liver.Journal of artificial organs : the official journal of the Japanese Society for Artificial Organs · 2025
    Article
  2. Article
  3. Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 2 countries.

Isabel M A BrüggenwirthDivision of Organ Transplantation, Department of Surgery, UMass Memorial Medical Center, University of Massachusetts, Worcester, MA.
Carolina MooreDivision of Organ Transplantation, Department of Surgery, UMass Memorial Medical Center, University of Massachusetts, Worcester, MA.
Paria MahboubDivision of Organ Transplantation, Department of Surgery, UMass Memorial Medical Center, University of Massachusetts, Worcester, MA.
Max F ThijssenDivision of Organ Transplantation, Department of Surgery, UMass Memorial Medical Center, University of Massachusetts, Worcester, MA.
Xiaofei EDepartment of Microbiology and Physiological Systems, University of Massachusetts Medical School, Worcester, MA.
Henri G D LeuveninkSurgical Research Laboratory, Department of Surgery, University Medical Center Groningen, The Netherlands.
Pranoti MandrekarDepartment of Internal Medicine, University of Massachusetts Medical School, Worcester, MA.
Xiaofei WangDepartment of Pathology, University of Massachusetts Medical School, Worcester, MA.
Timothy F KowalikDepartment of Microbiology and Physiological Systems, University of Massachusetts Medical School, Worcester, MA.
Robert J PorteSection of Hepato-Pancreato-Biliary Surgery and Liver Transplantation, Department of Surgery, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.
Paulo N MartinsDivision of Organ Transplantation, Department of Surgery, UMass Memorial Medical Center, University of Massachusetts, Worcester, MA.
UMass Memorial Medical Center · USUniversity of Massachusetts Chan Medical School · USUniversity Medical Center Groningen · NL

Funding

Role of heat shock protein 90 in alcoholic liver diseaseR01AA017986 · NIAAA · UNIV OF MASSACHUSETTS MED SCH WORCESTER · PI Pranoti Mandrekar · 2009 to 2026
$6.7M
Targeting proteotoxic stress responses in liver fibrosisR01AA025289 · NIAAA · UNIV OF MASSACHUSETTS MED SCH WORCESTER · PI MANDREKAR, PRANOTI · 2017 to 2021
$2.0M
NIAAA NIH HHS R01 AA017986NIAAA NIH HHS R01 AA025289
6 · The paper itself

Abstract

backgroundIt remains controversial if arterial perfusion in addition to portal vein perfusion during machine preservation improves liver graft quality. Comparative studies using both techniques are lacking. We studied the impact of using single or dual machine perfusion of donation after circulatory death rat livers. In addition, we analyzed the effect of pulsatile versus continuous arterial flow.

methodsDonation after circulatory death rat livers (n = 18) were preserved by 6 hours cold storage, followed by 1 hour subnormothermic machine perfusion (20°C, pressure of 40/5 mm Hg) and 2 hours ex vivo warm reperfusion (37°C, pressure of 80/11 mm Hg, 9% whole blood). Machine preservation was either through single portal vein perfusion (SP), dual pulsatile (DPP), or dual continuous perfusion (DCP) of the portal vein and hepatic artery. Hydrodynamics, liver function tests, histopathology, and expression of endothelial specific genes were assessed during 2 hours warm reperfusion.

resultsAt the end of reperfusion, arterial flow in DPP livers tended to be higher compared to DCP and SP grafts. However, this difference was not significant nor was better flow associated with better outcome. No differences in bile production or alanine aminotransferase levels were observed. SP livers had significantly lower lactate compared to DCP, but not DPP livers. Levels of Caspase-3 and tumor necrosis factor-α were similar between the groups. Expression of endothelial genes Krüppel-like-factor 2 and endothelial nitric oxide synthase tended to be higher in dual perfused livers, but no histological evidence of better preservation of the biliary endothelium or vasculature of the hepatic artery was observed.

conclusionsThis study shows comparable outcomes after using a dual or single perfusion approach during end-ischemic subnormothermic liver machine preservation.

Identifiers

PMID30534591
PMCPMC6233661
OpenAlexW2898158966

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.