ArticleSensors and actuators. B, Chemical2019
Microfluidic Exponential Rolling Circle Amplification for Sensitive microRNA Detection Directly from Biological Samples.
Article in Sensors and actuators. B, Chemical, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 65 citations in OpenAlex.
- Revolutionizing MDD diagnosis: the integrated role of circRNAs and biosensor technology.Molecular psychiatry · 2026Review
- Mitochondrial Metabolomics in Cancer: Mass Spectrometry-Based Approaches for Metabolic Rewiring Analysis and Therapeutic Discovery.Metabolites · 2025Review
- Anion recognition properties of acridine-urea conjugate in bulk and silica nanopore systems.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2025Article
- A one-pot isothermal Cas12-based assay for the sensitive detection of microRNAs.Nature biomedical engineering · 2023Article
- Simultaneous Detection of Tumor Derived Exosomal Protein-MicroRNA Pairs with an Exo-PROS Biosensor for Cancer Diagnosis.ACS nano · 2023Article
- Nucleotide detection mechanism and comparison based on low-dimensional materials: A review.Frontiers in bioengineering and biotechnology · 2023Review
- Silver nano-reporter enables simple and ultrasensitive profiling of microRNAs on a nanoflower-like microelectrode array on glass.Journal of nanobiotechnology · 2022Article
- Microfluidics for detection of exosomes and microRNAs in cancer: State of the art.Molecular therapy. Nucleic acids · 2022Review
- Microfluidic circulating reactor system for sensitive and automated duplex-specific nuclease-mediated microRNA detection.Talanta · 2021Article
- Elucidating Methods for Isolation and Quantification of Exosomes: A Review.Molecular biotechnology · 2021Review
- Ultrafast Detection of Exosomal RNAsACS applied nano materials · 2021Article
- Rolling Circle Amplification as a Universal Method for the Analysis of a Wide Range of Biological Targets.Russian journal of bioorganic chemistry · 2021Article
- Molecular and functional extracellular vesicle analysis using nanopatterned microchips monitors tumor progression and metastasis.Science translational medicine · 2020Article
- Sensor-Integrated Microfluidic Approaches for Liquid Biopsies Applications in Early Detection of Cancer.Sensors (Basel, Switzerland) · 2020Review
- Mitochondrial isolation: when size matters.Wellcome open research · 2020Review
- Circulating Exosomal miRNA as Diagnostic Biomarkers of Neurodegenerative Diseases.Frontiers in molecular neuroscience · 2020Review
- Molecular Approaches for Low-Cost Point-of-Care Pathogen Detection in Agriculture and Forestry.Frontiers in plant science · 2020Review
- Perspectives on the Role of Histone Modification in Breast Cancer Progression and the Advanced Technological Tools to Study Epigenetic Determinants of Metastasis.Frontiers in genetics · 2020Review
- Electrochemical lead(II) biosensor by using an ion-dependent split DNAzyme and a template-free DNA extension reaction for signal amplification.Mikrochimica acta · 2019Article
- The molecular mechanism underlying GABAergic dysfunction in nucleus accumbens of depression-like behaviours in mice.Journal of cellular and molecular medicine · 2019Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
There is an urgent need of sensitive bioanalytical platforms for sensitive and precise quantification of low-abundance microRNA targets in complex biological samples, including liquid biopsies of tumors. Many of current miRNA biosensing methods require laborious sample pretreatment procedures, including extraction of total RNA, which largely limits their biomedical and clinical applications. Herein we developed an integrated Microfluidic Exponential Rolling Circle Amplification (MERCA) platform for sensitive and specific detection of microRNAs directly in minimally processed samples. The MERCA system integrates and streamlines solid-phase miRNA isolation, miRNA-adapter ligation, and a dualphase exponential rolling circle amplification (eRCA) assay in one analytical workflow. By marrying the advantages of microfluidics in leveraging bioassay performance with the high sensitivity of eRCA, our method affords a remarkably low limit of detection at <10 zeptomole levels, with the ability to discriminate single-nucleotide difference. Using the MERCA chip, we demonstrated quantitative detection of miRNAs in total RNA, raw cell lysate, and cellderived exosomes. Comparing with the parallel TaqMan RT-qPCR measurements verified the adaptability of the MERCA system for detection of miRNA biomarkers in complex biological materials. In particular, high sensitivity of our method enables direct detection of low-level exosomal miRNAs in as few as 2 × 10
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.