Evidence map›Paper›PMID 30533061›Full record

ArticlePloS one2018

Assessment and verification of commercially available pressure cookers for laboratory sterilization.

Vaille A Swenson, Amanda D Stacy, Michael O Gaylor, Blake Ushijima, Benjamin Philmus, Loralyn M Cozy, Nina M Videau, Patrick Videau

Abstract read
In one paragraph

Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed, 2 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 2 syntheses or guidelines pooled it.

  1. Pooled it
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  3. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Vaille A SwensonBiology Department, Dakota State University, Madison, South Dakota, United States of America.
Amanda D StacyDepartment of Biology, Southern Oregon University, Oregon, Ashland, United States of America.
Michael O GaylorChemistry Department, Dakota State University, Madison, South Dakota, United States of America.
Blake UshijimaCarlson College of Veterinary Medicine, Oregon State University, Corvallis, Oregon, United States of America.
Benjamin PhilmusDepartment of Pharmaceutical Sciences, College of Pharmacy, Oregon State University, Corvallis, Oregon, United States of America.
Loralyn M CozyDepartment of Biology, Illinois Wesleyan University, Bloomington, Illinois, United States of America.
Nina M VideauBowman Gray Center for Medical Education, Wake Forest University, Winston-Salem, North Carolina, United States of America.
Patrick VideauBiology Department, Dakota State University, Madison, South Dakota, United States of America.ORCID 0000-0003-2686-845X

Funding

A workflow for identification of gene clusters and heterologous expression of cyanobacterial natural productsR15GM117541 · NIGMS · OREGON STATE UNIVERSITY · PI PHILMUS, BENJAMIN · 2016 to 2016
$422k
NIGMS NIH HHS R15 GM117541
6 · The paper itself

Abstract

Laboratory science requires careful maintenance of sterile reagents and tools as well as the sterilization of waste prior to disposal. However, steam autoclaves typically used for this purpose may not be readily accessible to everyone in the scientific community, such as K-12 teachers, researchers in the field, students in under-funded laboratories, or persons in the developing world who lack funding and resources. This work examines the use of commercial electric pressure cookers as an alternative method for the sterilization of media, instruments, and waste. Four commonly available brands of pressure cooker were tested for their ability to sterilize microbiological media, a variety of metal instruments, and high-titer microbial cultures. All four pressure cookers were able to sterilize these starting materials as well as a range of microbial types, including Gram-positive bacteria, Gram-negative bacteria, filamentous fungi, unicellular fungi, and mixed environmental samples. Only the Instant Pot, however, was able to sterilize autoclave tester ampoules of Geobacillus stearothermophilus spores. These results suggest that, depending on the nature of the work undertaken, store-bought pressure cookers can be an appropriate substitute for commercial autoclaves. Their adoption may also help increase the accessibility of science to a broader range of investigators.

Indexed as

Culture MediaEquipment ContaminationFungiGram-Negative BacteriaGram-Positive BacteriaLaboratoriesPressureSpores, BacterialSterilizationCulture Media

Identifiers

PMID30533061
PMCPMC6289433

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.