ArticleJournal of the American Chemical Society2019
Fluorescence Enhancement of a Microbial Rhodopsin via Electronic Reprogramming.
Article in Journal of the American Chemical Society, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 48 citations in OpenAlex.
- Natural and engineered charge-transfer and diradical state mixing and retinal chromophore dynamics.Physical chemistry chemical physics : PCCP · 2026Review
- Article
- Retinal to Retinal Energy Transfer in a Bistable Microbial Rhodopsin Dimer.Journal of the American Chemical Society · 2025Article
- Ultrafast terahertz Stark spectroscopy reveals the excited-state dipole moments of retinal in bacteriorhodopsin.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Voltage Imaging with Engineered Proton-Pumping Rhodopsins: Insights from the Proton Transfer Pathway.ACS physical chemistry Au · 2023Review
- Retinal chromophore charge delocalization and confinement explain the extreme photophysics of Neorhodopsin.Nature communications · 2022Article
- On the fluorescence enhancement of arch neuronal optogenetic reporters.Nature communications · 2022Article
- QuasAr Odyssey: the origin of fluorescence and its voltage sensitivity in microbial rhodopsins.Nature communications · 2022Article
- Bidirectional Photochemistry of Antarctic Microbial Rhodopsin: Emerging Trend of Ballistic Photoisomerization from the 13-The journal of physical chemistry letters · 2022Article
- Fragment Localized Molecular Orbitals.Journal of chemical theory and computation · 2022Article
- Evolution of the Automatic Rhodopsin Modeling (ARM) Protocol.Topics in current chemistry (Cham) · 2022Review
- Energetic Basis and Design of Enzyme Function Demonstrated Using GFP, an Excited-State Enzyme.Journal of the American Chemical Society · 2022Article
- Rhodopsins: An Excitingly Versatile Protein Species for Research, Development and Creative Engineering.Frontiers in chemistry · 2022Review
- Far-Red Absorbing Rhodopsins, Insights From Heterodimeric Rhodopsin-Cyclases.Frontiers in molecular biosciences · 2021Review
- Dual Photoisomerization on Distinct Potential Energy Surfaces in a UV-Absorbing Rhodopsin.Journal of the American Chemical Society · 2020Article
- Web-ARM: A Web-Based Interface for the Automatic Construction of QM/MM Models of Rhodopsins.Journal of chemical information and modeling · 2020Article
- Electrostatic control of photoisomerization pathways in proteins.Science (New York, N.Y.) · 2020Article
- Multistate Multiconfiguration Quantum Chemical Computation of the Two-Photon Absorption Spectra of Bovine Rhodopsin.The journal of physical chemistry letters · 2019Article
- a-ARM: Automatic Rhodopsin Modeling with Chromophore Cavity Generation, Ionization State Selection, and External Counterion Placement.Journal of chemical theory and computation · 2019Article
- Two-State, Three-Mode Parametrization of the Force Field of a Retinal Chromophore Model.The journal of physical chemistry. A · 2019Article
Corrections and comments
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Authors and funding
11 authors at 6 institutions in 6 countries.
Funding
Abstract
The engineering of microbial rhodopsins with enhanced fluorescence is of great importance in the expanding field of optogenetics. Here we report the discovery of two mutants (W76S/Y179F and L83Q) of a sensory rhodopsin from the cyanobacterium Anabaena PCC7120 with opposite fluorescence behavior. In fact, while W76S/Y179F displays, with respect to the wild-type protein, a nearly 10-fold increase in red-light emission, the second is not emissive. Thus, the W76S/Y179F, L83Q pair offers an unprecedented opportunity for the investigation of fluorescence enhancement in microbial rhodopsins, which is pursued by combining transient absorption spectroscopy and multiconfigurational quantum chemistry. The results of such an investigation point to an isomerization-blocking electronic effect as the direct cause of instantaneous (subpicosecond) fluorescence enhancement.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.