ArticlePloS one2018
Filter paper-based spin column method for cost-efficient DNA or RNA purification.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Nucleic acids in medicinal plants and their pharmaceutical potential.Extracellular vesicles and circulating nucleic acids · 2026Review
- A novel and simple workflow for investigating Mycoplasma spp. contamination in cell cultures.Folia microbiologica · 2025Article
- Development of Rapid Detection Methods forInternational journal of molecular sciences · 2024Article
- A high-performance polymer composite column for coronavirus nucleic acid purification.Scientific reports · 2024Article
- Analysis of Skin Regeneration and Barrier-Improvement Efficacy of Polydeoxyribonucleotide Isolated from Panax Ginseng (C.A. Mey.) Adventitious Root.Molecules (Basel, Switzerland) · 2023Article
- Handheld Purification-Free Nucleic Acid Testing Device for Point-of-Need Detection of Malaria from Whole Blood.ACS sensors · 2023Article
- An Efficient and Simple Method for Collecting Haemolymph of Cerambycidae (Insecta: Coleoptera) Adults.Insects · 2022Article
- Use of polypyrrole-polystyrene membranes for extracting DNA from plant tissues.Biomaterials and biosystems · 2022Article
- Improved DNA extraction on bamboo paper and cotton is tightly correlated with their crystallinity and hygroscopicity.PloS one · 2022Article
- Homoeologous chromosome exchange explains the creation of a QTL affecting soil-borne pathogen resistance in tobacco.Plant biotechnology journal · 2022Article
- Sequence-selective purification of biological RNAs using DNA nanoswitches.Cell reports methods · 2021Article
- Association between genetic variations in carbamoyl-phosphate synthetase gene and persistent neonatal pulmonary hypertension.European journal of pediatrics · 2021Article
- Rapid (30-second), equipment-free purification of nucleic acids using easy-to-make dipsticks.Nature protocols · 2020Article
- CRISPR-Cas fluorescent cleavage assay coupled with recombinase polymerase amplification for sensitive and specific detection ofBiotechnology reports (Amsterdam, Netherlands) · 2020Article
- Strategies for reducing per-sample costs in target capture sequencing for phylogenomics and population genomics in plants.Applications in plant sciences · 2020Review
- Application of a cost-effective DNA extraction protocol for screening transgenic and CRISPR-edited primary goat cells.PloS one · 2020Article
- Molecular Detection Assays for Rapid Field-Detection of Rice Sheath Blight.Frontiers in plant science · 2020Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We describe herein a method of recharging used commercial spin columns or assembling homemade spin columns using filter paper as binding material for cost-effective, low throughput nucleic acid purification. The efficiency of filter paper-based spin columns was evaluated for purification of nucleic acids from various sources. Following protocols of commercial kits, we found filter paper to be a useful binding material for purification of nucleic acids, including plant genomic DNA, plant total RNA, PCR products, and DNA from agarose gels. However, filter paper has a weak binding affinity to plasmid DNA in tested miniprep protocols. Protocols for the use of filter paper recharged spin columns or homemade spin columns for low throughput purification of plant genomic DNA and total RNA with unused commercial kit buffers or less expensive homemade buffers are presented.
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