ArticlePLoS genetics2018
RNA-on-X 1 and 2 in Drosophila melanogaster fulfill separate functions in dosage compensation.
Article in PLoS genetics, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 27 citations in OpenAlex.
- Dosage compensation defects due to roX RNA loss are rescued by recalibration of X/autosome stoichiometry.Nucleic acids research · 2026Article
- Promiscuous RNA editing in lncRNARNA (New York, N.Y.) · 2025Article
- A noncanonical role of roX RNAs in autosomal epigenetic repression.Nature communications · 2025Article
- N-terminus ofeLife · 2024Article
- Article
- Transcriptomic Analysis ofInternational journal of molecular sciences · 2023Article
- Integrating massive RNA-seq data to elucidate transcriptome dynamics in Drosophila melanogaster.Briefings in bioinformatics · 2023Article
- Pleiotropic fitness effects of the lncRNA Uhg4 in Drosophila melanogaster.BMC genomics · 2022Article
- Cis- and trans-regulation by histone H4 basic patch R17/R19 in metazoan development.Open biology · 2022Article
- Article
- Dosage Compensation inInternational journal of molecular sciences · 2022Review
- Modulation of RNA stability regulates gene expression in two opposite ways: through buffering of RNA levels upon global perturbations and by supporting adapted differential expression.Nucleic acids research · 2022Article
- The role of H3K36 methylation and associated methyltransferases in chromosome-specific gene regulation.Science advances · 2021Article
- DMRT1 gene disruption alone induces incomplete gonad feminization in chicken.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021Article
- Long Non-Coding RNAs in Insects.Animals : an open access journal from MDPI · 2021Review
- Article
- Article
- Hi-C guided assemblies reveal conserved regulatory topologies on X and autosomes despite extensive genome shuffling.Genes & development · 2019Article
- Insights into the Functions of LncRNAs inInternational journal of molecular sciences · 2019Review
- MAPCap allows high-resolution detection and differential expression analysis of transcription start sites.Nature communications · 2019Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In Drosophila melanogaster, the male-specific lethal (MSL) complex plays a key role in dosage compensation by stimulating expression of male X-chromosome genes. It consists of MSL proteins and two long noncoding RNAs, roX1 and roX2, that are required for spreading of the complex on the chromosome and are redundant in the sense that loss of either does not affect male viability. However, despite rapid evolution, both roX species are present in diverse Drosophilidae species, raising doubts about their full functional redundancy. Thus, we have investigated consequences of deleting roX1 and/or roX2 to probe their specific roles and redundancies in D. melanogaster. We have created a new mutant allele of roX2 and show that roX1 and roX2 have partly separable functions in dosage compensation. In larvae, roX1 is the most abundant variant and the only variant present in the MSL complex when the complex is transmitted (physically associated with the X-chromosome) in mitosis. Loss of roX1 results in reduced expression of the genes on the X-chromosome, while loss of roX2 leads to MSL-independent upregulation of genes with male-biased testis-specific transcription. In roX1 roX2 mutant, gene expression is strongly reduced in a manner that is not related to proximity to high-affinity sites. Our results suggest that high tolerance of mis-expression of the X-chromosome has evolved. We propose that this may be a common property of sex-chromosomes, that dosage compensation is a stochastic process and its precision for each individual gene is regulated by the density of high-affinity sites in the locus.
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