ArticleScientific reports2018
Quantification of protein mobility and associated reshuffling of cytoplasm during chemical fixation.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 48 citations in OpenAlex.
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- Quantitative Characterization of Microtubule Ultrastructure Based on Single-Molecule Localization Microscopy.Cytoskeleton (Hoboken, N.J.) · 2026Article
- Nanoscale architecture and dynamics of CaJournal of molecular and cellular cardiology plus · 2025Article
- Raman microscopy of cryofixed biological specimens for high-resolution and high-sensitivity chemical imaging.Science advances · 2024Article
- CaCommunications biology · 2024Article
- An aldehyde-crosslinking mitochondrial probe for STED imaging in fixed cells.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Correlative single-cell hard X-ray computed tomography and X-ray fluorescence imaging.Communications biology · 2024Article
- The use of scanning electron microscopy and fixation methods to evaluate the interaction of blood with the surfaces of medical devices.Scientific reports · 2024Article
- Structural brain preservation: a potential bridge to future medical technologies.Frontiers in medical technology · 2024Article
- Handling Difficult Cryo-ET Samples: A Study with Primary Neurons from Drosophila melanogaster.Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada · 2023Article
- Non-invasive investigation of the morphology and optical properties of the upside-down jellyfishProceedings. Biological sciences · 2023Article
- Preserving extracellular space for high-quality optical and ultrastructural studies of whole mammalian brains.Cell reports methods · 2023Article
- Visualizing Protein Localizations in Fixed Cells: Caveats and the Underlying Mechanisms.The journal of physical chemistry. B · 2023Review
- Article
- HS1 deficiency protects against sepsis by attenuating neutrophil-inflicted lung damage.European journal of cell biology · 2022Article
- Ultrarapid cryo-arrest of living cells on a microscope enables multiscale imaging of out-of-equilibrium molecular patterns.Science advances · 2021Article
- Translocation of chromatin proteins to nucleoli-The influence of protein dynamics on post-fixation localization.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2021Article
- Algae to angiosperms: Autofluorescence for rapid visualization of plant anatomy among diverse taxa.Applications in plant sciences · 2021Article
- An optimized fixation method containing glyoxal and paraformaldehyde for imaging nuclear bodies.RNA (New York, N.Y.) · 2021Article
- The Combination of Paraformaldehyde and Glutaraldehyde Is a Potential Fixative for Mitochondria.Biomolecules · 2021Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To understand cellular functionalities, it is essential to unravel spatio-temporal patterns of molecular distributions and interactions within living cells. The technological progress in fluorescence microscopy now allows in principle to measure these patterns with sufficient spatial resolution. However, high resolution imaging comes with long acquisition times and high phototoxicity. Therefore, physiological live cell imaging is often unfeasible and chemical fixation is employed. Yet, fixation methods have not been rigorously investigated, in terms of pattern preservation, at the resolution at which cells can now be imaged. A key parameter for this is the time required until fixation is complete. During this time, cells are under unphysiological conditions and patterns decay. We demonstrate here that formaldehyde fixation takes more than one hour for cytosolic proteins in cultured cells. Other small aldehydes, glyoxal and acrolein, did not perform better. Associated with this, we found a distinct displacement of proteins and lipids, including their loss from cells. Fixations using glutaraldehyde were faster than four minutes and retained most cytoplasmic proteins. Surprisingly, autofluorescence produced by glutaraldehyde was almost completely absent with supplementary addition of formaldehyde without compromising fixation speed. These findings indicate, which cellular processes can actually be reliably imaged after a certain chemical fixation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.