ArticleEmerging microbes & infections2018
Replication of MERS and SARS coronaviruses in bat cells offers insights to their ancestral origins.
Article in Emerging microbes & infections, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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34 citing papers in PubMed, 42 citations in OpenAlex.
- Chiropteran (Animals : an open access journal from MDPI · 2026Article
- Addressing the zoonotic threat of merbecoviruses.Nature microbiology · 2026Review
- Clade C MERS-CoV camel strains vary in protease utilization during viral entry.bioRxiv : the preprint server for biology · 2025Article
- From zoonotic spillover to endemicity: the broad determinants of human coronavirus tropism.mBio · 2025Review
- Infection of animal coronaviruses into a bat cell line from the kidney of Pipistrellus abramus.The Journal of veterinary medical science · 2025Article
- Structures and receptor binding activities of merbecovirus spike proteins reveal key signatures for human DPP4 adaptation.Science advances · 2025Article
- ACE2 from Pipistrellus abramus bats is a receptor for HKU5 coronaviruses.Nature communications · 2025Article
- Article
- [What do we know about the origin of COVID-19 three years later?]Revista clinica espanola · 2023Review
- What do we know about the origin of COVID-19 three years later?Revista clinica espanola · 2023Review
- Bats-associated beta-coronavirus detection and characterization: First report from Pakistan.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2023Article
- Full-Length Transcriptome ofInternational journal of molecular sciences · 2022Article
- Generation of a VeroE6 Pgp gene knock out cell line and its use in SARS-CoV-2 antiviral study.Antiviral research · 2022Article
- Molecular aspects of Omicron, vaccine development, and recombinant strain XE: A review.Journal of medical virology · 2022Review
- A Look inside the Replication Dynamics of SARS-CoV-2 in Blyth's Horseshoe Bat (Microbiology spectrum · 2022Article
- Interspecies Jumping of Bat Coronaviruses.Viruses · 2021Review
- Sequence and phylogentic analysis of MERS-CoV in Saudi Arabia, 2012-2019.Virology journal · 2021Article
- Isolation of MERS-related coronavirus from lesser bamboo bats that uses DPP4 and infects human-DPP4-transgenic mice.Nature communications · 2021Article
- Gordian Knot: Gastrointestinal lesions caused by three highly pathogenic coronaviruses from SARS-CoV and MERS-CoV to SARS-CoV-2.European journal of pharmacology · 2021Review
- Differential Tropism of SARS-CoV and SARS-CoV-2 in Bat Cells.Emerging infectious diseases · 2020Article
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Authors and funding
14 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Previous findings of Middle East Respiratory Syndrome coronavirus (MERS-CoV)-related viruses in bats, and the ability of Tylonycteris-BatCoV HKU4 spike protein to utilize MERS-CoV receptor, human dipeptidyl peptidase 4 hDPP4, suggest a bat ancestral origin of MERS-CoV. We developed 12 primary bat cell lines from seven bat species, including Tylonycteris pachypus, Pipistrellus abramus and Rhinolophus sinicus (hosts of Tylonycteris-BatCoV HKU4, Pipistrellus-BatCoV HKU5, and SARS-related-CoV respectively), and tested their susceptibilities to MERS-CoVs, SARS-CoV, and human coronavirus 229E (HCoV-229E). Five cell lines, including P. abramus and R. sinicus but not T. pachypus cells, were susceptible to human MERS-CoV EMC/2012. However, three tested camel MERS-CoV strains showed different infectivities, with only two strains capable of infecting three and one cell lines respectively. SARS-CoV can only replicate in R. sinicus cells, while HCoV-229E cannot replicate in any bat cells. Bat dipeptidyl peptidase 4 (DPP4) sequences were closely related to those of human and non-human primates but distinct from dromedary DPP4 sequence. Critical residues for binding to MERS-CoV spike protein were mostly conserved in bat DPP4. DPP4 was expressed in the five bat cells susceptible to MERS-CoV, with significantly higher mRNA expression levels than those in non-susceptible cells (P = 0.0174), supporting that DPP4 expression is critical for MERS-CoV infection in bats. However, overexpression of T. pachypus DPP4 failed to confer MERS-CoV susceptibility in T. pachypus cells, suggesting other cellular factors in determining viral replication. The broad cellular tropism of MERS-CoV should prompt further exploration of host diversity of related viruses to identify its ancestral origin.
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