Evidence map›Paper›PMID 30531986›Full record

ArticleScientific reports2018

Development of real-time reverse transcription recombinase polymerase amplification (RPA) for rapid detection of peste des petits ruminants virus in clinical samples and its comparison with real-time PCR test.

Yuanli Li, Lin Li, Xiaoxu Fan, Yanli Zou, Yongqiang Zhang, Qinghua Wang, Chengyou Sun, Shude Pan, Xiaodong Wu, Zhiliang Wang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed.

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  16. "FastCheckViruses · 2020
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Yuanli LiOIE Reference Laboratory for Peste des Petits Ruminants, National Research Center for Exotic Animal Diseases, China Animal Health and Epidemiology Center, Qingdao, Shandong, P. R. China.
Lin LiOIE Reference Laboratory for Peste des Petits Ruminants, National Research Center for Exotic Animal Diseases, China Animal Health and Epidemiology Center, Qingdao, Shandong, P. R. China.
Xiaoxu FanOIE Reference Laboratory for Peste des Petits Ruminants, National Research Center for Exotic Animal Diseases, China Animal Health and Epidemiology Center, Qingdao, Shandong, P. R. China.
Yanli ZouOIE Reference Laboratory for Peste des Petits Ruminants, National Research Center for Exotic Animal Diseases, China Animal Health and Epidemiology Center, Qingdao, Shandong, P. R. China.
Yongqiang ZhangOIE Reference Laboratory for Peste des Petits Ruminants, National Research Center for Exotic Animal Diseases, China Animal Health and Epidemiology Center, Qingdao, Shandong, P. R. China.
Qinghua WangOIE Reference Laboratory for Peste des Petits Ruminants, National Research Center for Exotic Animal Diseases, China Animal Health and Epidemiology Center, Qingdao, Shandong, P. R. China.
Chengyou SunOIE Reference Laboratory for Peste des Petits Ruminants, National Research Center for Exotic Animal Diseases, China Animal Health and Epidemiology Center, Qingdao, Shandong, P. R. China.
Shude PanCollege of Animal Husbandry and Veterinary Medicine, Shenyang Agricultural University, Shenyang, Liaoning, P. R. China.
Xiaodong WuOIE Reference Laboratory for Peste des Petits Ruminants, National Research Center for Exotic Animal Diseases, China Animal Health and Epidemiology Center, Qingdao, Shandong, P. R. China. wuxiaodong@cahec.cn.
Zhiliang WangOIE Reference Laboratory for Peste des Petits Ruminants, National Research Center for Exotic Animal Diseases, China Animal Health and Epidemiology Center, Qingdao, Shandong, P. R. China. wangzhiliang@cahec.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Peste des petits ruminants (PPR), caused by small ruminant morbillivirus (SRMV), formerly called peste des petits ruminants virus (PPRV), is one of the most important pathogens in small ruminants, and has tremendous negative economic impact on the sheep industry worldwide. Current detection of PPRV in clinical samples mainly relies on real-time RT-PCR. Particularly, samples collected from rural area require highly equipped laboratories for screening. A rapid, real-time reverse-transcription recombinase polymerase amplification assay (RT-RPA), employing primers and exo probe, was thus developed to perform at 42 °C for 20 min, and the detection limit at 95% probability was 14.98 copies per reaction and 0.326 TCID

Indexed as

AnimalsDistemper Virus, CanineDNA PrimersFoot-and-Mouth Disease VirusGoat DiseasesGoatsNucleic Acid Amplification TechniquesPeste-des-Petits-RuminantsPeste-des-petits-ruminants virusReal-Time Polymerase Chain ReactionRecombinasesReverse TranscriptionRNA, ViralSensitivity and SpecificitySheepSheep DiseasesDNA PrimersRecombinasesRNA, Viral

Identifiers

PMID30531986
PMCPMC6288080

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.