ArticleNature chemical biology2019
A DNA-based fluorescent reporter maps HOCl production in the maturing phagosome.
Article in Nature chemical biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 78 citations in OpenAlex.
- High-throughput measurement and prediction of the i-motif DNA stability landscape.Nucleic acids research · 2026Article
- Organelles harbour pH gradients.Research square · 2026Article
- Advances in endogenous hypochlorous acid-mediated regulation of tumor cell fates.Frontiers in cell and developmental biology · 2026Review
- Organelles harbour pH gradients.bioRxiv : the preprint server for biology · 2025Article
- Targeted imaging of lysosomal zinc ions with a tetrahedral DNA framework fluorescent reporter.National science review · 2024Article
- Leveraging Chlorination-Based Mechanism for Resolving Subcellular Hypochlorous Acid.bioRxiv : the preprint server for biology · 2024Article
- Quantitative Chemical Imaging of Organelles.Accounts of chemical research · 2024Article
- Fe-doped carbon dots: a novel fluorescent nanoprobe for cellular hypochlorous acid imaging.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2024Article
- Light and endogenous enzyme triggered plasmonic antennas for accurate subcellular molecular imaging with enhanced spatial resolution.Chemical science · 2024Article
- Fluorescent/Phosphorescent Dual-Emissive Probe for Simultaneous and Independent Sensing of Intracellular Hypochlorite and pH via Time-Resolved Luminescence Imaging.Chemical & biomedical imaging · 2023Article
- The evolution of organellar calcium mapping technologies.Cell calcium · 2022Review
- Recent advance in dual-functional luminescent probes for reactive species and common biological ions.Analytical and bioanalytical chemistry · 2022Review
- Development of the DNA-based biosensors for high performance in detection of molecular biomarkers: More rapid, sensitive, and universal.Biosensors & bioelectronics · 2022Review
- Stress response decay with aging visualized using a dual-channel logic-based fluorescent probe.Chemical science · 2021Article
- Tubular lysosomes harbor active ion gradients and poise macrophages for phagocytosis.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Article
- Rational design of guiding elements to control folding topology in i-motifs with multiple quadruplexes.Nanoscale · 2021Article
- Quantifying phagosomal HOCl at single immune-cell resolution.Methods in cell biology · 2021Article
- Quantitative Imaging of BiochemistryACS central science · 2020Review
- Combining Qdot Nanotechnology and DNA Nanotechnology for Sensitive Single-Cell Imaging.Advanced materials (Deerfield Beach, Fla.) · 2020Article
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7 authors at 1 institution in 1 country.
Funding
Abstract
Phagocytes destroy pathogens by trapping them in a transient organelle called the phagosome, where they are bombarded with reactive oxygen species (ROS) and reactive nitrogen species (RNS). Imaging reactive species within the phagosome would directly reveal the chemical dynamics underlying pathogen destruction. Here we introduce a fluorescent, DNA-based combination reporter, cHOClate, which simultaneously images hypochlorous acid (HOCl) and pH quantitatively. Using cHOClate targeted to phagosomes in live cells, we successfully map phagosomal production of a specific ROS, HOCl, as a function of phagosome maturation. We found that phagosomal acidification was gradual in macrophages and upon completion, HOCl was released in a burst. This revealed that phagosome-lysosome fusion was essential not only for phagosome acidification, but also for providing the chloride necessary for myeloperoxidase activity. This method can be expanded to image several kinds of ROS and RNS and be readily applied to identify how resistant pathogens evade phagosomal killing.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.