Evidence map›Paper›PMID 30530245›Full record

ArticleBiomaterials2019

Microfluidic-enabled bottom-up hydrogels from annealable naturally-derived protein microbeads.

Amir Sheikhi, Joseph de Rutte, Reihaneh Haghniaz, Outman Akouissi, Alireza Sohrabi, Dino Di Carlo, Ali Khademhosseini

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomaterials, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 77 papers.

0numbers the graph read from it
0cells of the map it votes in
77citing papers in PubMed
6.1field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

77 citing papers in PubMed, 172 citations in OpenAlex.

  1. Article
  2. Review
  3. Cryopreservative Bioink Enables Direct Bioprinting of Adherent Cells.Advanced materials (Deerfield Beach, Fla.) · 2026
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  7. Review
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  9. Gelatin Methacrylate Macroporous Cell Scaffold Fabrication via One-Pot Aqueous Two-Phase Separation.Journal of biomedical materials research. Part B, Applied biomaterials · 2025
    Article
  10. Article
  11. Review
  12. Article
  13. Article
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  15. Article
  16. Article
  17. Review
  18. Article
  19. Gelatin maleimide microgels for hematopoietic progenitor cell encapsulation.Journal of biomedical materials research. Part A · 2024
    Article
  20. Article

17 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 2 countries.

Amir SheikhiDepartment of Bioengineering, University of California - Los Angeles, 410 Westwood Plaza, Los Angeles, CA 90095, USA; Center for Minimally Invasive Therapeutics (C-MIT), University of California - Los Angeles, 570 Westwood Plaza, Los Angeles, CA 90095, USA; California NanoSystems Institute (CNSI), University of California - Los Angeles, 570 Westwood Plaza, Los Angeles, CA 90095, USA.
Joseph de RutteDepartment of Bioengineering, University of California - Los Angeles, 410 Westwood Plaza, Los Angeles, CA 90095, USA.
Reihaneh HaghniazDepartment of Bioengineering, University of California - Los Angeles, 410 Westwood Plaza, Los Angeles, CA 90095, USA; Center for Minimally Invasive Therapeutics (C-MIT), University of California - Los Angeles, 570 Westwood Plaza, Los Angeles, CA 90095, USA; California NanoSystems Institute (CNSI), University of California - Los Angeles, 570 Westwood Plaza, Los Angeles, CA 90095, USA.
Outman AkouissiDepartment of Bioengineering, University of California - Los Angeles, 410 Westwood Plaza, Los Angeles, CA 90095, USA; Center for Minimally Invasive Therapeutics (C-MIT), University of California - Los Angeles, 570 Westwood Plaza, Los Angeles, CA 90095, USA; California NanoSystems Institute (CNSI), University of California - Los Angeles, 570 Westwood Plaza, Los Angeles, CA 90095, USA.
Alireza SohrabiDepartment of Bioengineering, University of California - Los Angeles, 410 Westwood Plaza, Los Angeles, CA 90095, USA.
Dino Di CarloDepartment of Bioengineering, University of California - Los Angeles, 410 Westwood Plaza, Los Angeles, CA 90095, USA; California NanoSystems Institute (CNSI), University of California - Los Angeles, 570 Westwood Plaza, Los Angeles, CA 90095, USA; Jonsson Comprehensive Cancer Centre, University of California - Los Angeles, 10833 Le Conte Ave, Los Angeles, CA 90024, USA. Electronic address: dicarlo@g.ucla.edu.
Ali KhademhosseiniDepartment of Bioengineering, University of California - Los Angeles, 410 Westwood Plaza, Los Angeles, CA 90095, USA; Center for Minimally Invasive Therapeutics (C-MIT), University of California - Los Angeles, 570 Westwood Plaza, Los Angeles, CA 90095, USA; California NanoSystems Institute (CNSI), University of California - Los Angeles, 570 Westwood Plaza, Los Angeles, CA 90095, USA; Jonsson Comprehensive Cancer Centre, University of California - Los Angeles, 10833 Le Conte Ave, Los Angeles, CA 90024, USA; Department of Radiological Sciences, David Geffen School of Medicine, University of California - Los Angeles, 10833 Le Conte Ave, Los Angeles, CA 90095, USA; Department of Chemical and Biomolecular Engineering, University of California - Los Angeles, 5531 Boelter Hall, Los Angeles, CA 90095, USA; Department of Bioindustrial Technologies, College of Animal Bioscience and Technology, Konkuk University, Seoul, 143-701, Republic of Korea. Electronic address: khademh@ucla.edu.
University of California, Los Angeles · USCalifornia NanoSystems Institute · USUCLA Jonsson Comprehensive Cancer Center

Funding

Microengineered Osteons for Bone Tissue EngineeringR01AR057837 · NIAMS · UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON · PI YANG, YUNZHI · 2010 to 2018
$5.1M
Hemorrhage control in the irreversible anticoagulated patientR01HL137193 · NHLBI · MAYO CLINIC ARIZONA · PI OKLU, RAHMI · 2017 to 2021
$3.0M
Rapid 3D bioprinting of biomimetic vascularized tissue constructsR01EB021857 · NIBIB · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI CHEN, SHAOCHEN, DOKMECI, MEHMET REMZI · 2016 to 2019
$2.1M
CIHRNHLBI NIH HHS R01 HL137193NIAMS NIH HHS R01 AR057837NIBIB NIH HHS R01 EB021857
6 · The paper itself

Abstract

Naturally-derived proteins, such as collagen, elastin, fibroin, and gelatin (denatured collagen) hold a remarkable promise for tissue engineering and regenerative medicine. Gelatin methacryloyl (GelMA), synthesized from the methacryloyl modification of gelatin, mimicking the structure of extracellular matrix, has widely been used as a universal multi-responsive scaffold for a broad spectrum of applications, spanning from cell therapy to bioprinting and organoid development. Despite the widespread applications of GelMA, coupled stiffness and porosity has inhibited its applications in 3D cellular engineering wherein a stiff scaffold with large pores is demanded (e.g., at concentrations >10 wt%). Taking advantage of the orthogonal thermo-chemical responsivity of GelMA, we have developed microfluidic-assisted annealable GelMA beads, that are first stabilized by temperature-mediated physical crosslinking, flowed to form a scaffold structure, and then chemically annealed using light to fabricate novel bead-based 3D GelMA scaffolds with high mechanical resilience. We show how beaded GelMA (B-GelMA) provides a self-standing microporous environment with an orthogonal void fraction and stiffness, promoting cell adhesion, proliferation, and rapid 3D seeding at a high polymer concentration (∼20 wt%) that would otherwise be impossible for bulk GelMA. B-GelMA, decorated with methacryloyl and arginylglycylaspartic acid (RGD) peptide motifs, does not require additional functionalization for annealing and cell adhesion, providing a versatile biorthogonal platform with orthogonal stiffness and porosity for a myriad of biomedical applications. This technology provides a universal method to convert polymeric materials with orthogonal physico-chemical responsivity to modular platforms, opening a new horizon for converting bulk hydrogels to beaded hydrogels (B-hydrogels) with decoupled porosity and stiffness.

Indexed as

Biocompatible MaterialsEquipment DesignGelatinHumansHuman Umbilical Vein Endothelial CellsHydrogelsMicrofluidic Analytical TechniquesPorosityProteinsBiocompatible MaterialsGelatinHydrogelsProteins3D cell seedingGelatin methacryloyl (GelMA)MicrobeadsMicroporous scaffoldsModular hydrogelsParticle gels

Identifiers

PMID30530245
PMCPMC6400213
OpenAlexW2900051774

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.