Evidence map›Paper›PMID 30529623›Full record

ArticleEcotoxicology and environmental safety2019

Biocorona formation contributes to silver nanoparticle induced endoplasmic reticulum stress.

Indushekhar Persaud, Jonathan H Shannahan, Achyut J Raghavendra, Nasser B Alsaleh, Ramakrishna Podila, Jared M Brown

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Ecotoxicology and environmental safety, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed, 1 pooled it
2.1field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 1 synthesis or guideline pooled it, 39 citations in OpenAlex.

  1. Analyzing Molecular Determinants of Nanodrugs' Cytotoxic Effects.International journal of molecular sciences · 2025
    Pooled it
  2. Review
  3. Review
  4. Article
  5. Article
  6. Article
  7. Review
  8. Article
  9. Review
  10. Review
  11. Review
  12. Article
  13. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Indushekhar PersaudDepartment of Pharmaceutical Sciences, Skaggs School of Pharmacy and Pharmaceutical Sciences, University of Colorado, Aurora, CO 80045, USA.
Jonathan H ShannahanSchool of Health Sciences, College of Health and Human Sciences, Purdue University, West Lafayette, IN 47907, USA.
Achyut J RaghavendraDepartment of Physics and Astronomy, Clemson University, Clemson, SC 29634, USA; Clemson Nanomaterials Center and COMSET, Clemson University, Anderson, SC 29625, USA.
Nasser B AlsalehDepartment of Pharmaceutical Sciences, Skaggs School of Pharmacy and Pharmaceutical Sciences, University of Colorado, Aurora, CO 80045, USA.
Ramakrishna PodilaDepartment of Physics and Astronomy, Clemson University, Clemson, SC 29634, USA; Clemson Nanomaterials Center and COMSET, Clemson University, Anderson, SC 29625, USA.
Jared M BrownDepartment of Pharmaceutical Sciences, Skaggs School of Pharmacy and Pharmaceutical Sciences, University of Colorado, Aurora, CO 80045, USA. Electronic address: jared.brown@ucdenver.edu.
University of Colorado Anschutz Medical Campus · USClemson University · USPurdue University West Lafayette · US

Funding

Mechanisms of non-IgE Mast Cell Activation by Environmental ParticulatesR01ES019311 · NIEHS · UNIVERSITY OF COLORADO DENVER · PI BROWN, JARED MICHAEL · 2010 to 2024
$7.0M
Predicting the Toxicity of Nanomaterials by a Transforming Protein CoronaR15ES022766 · NIEHS · CLEMSON UNIVERSITY · PI RAO, APPARAO · 2014 to 2014
$445k
NIEHS NIH HHS R01 ES019311NIEHS NIH HHS R15 ES022766
6 · The paper itself

Abstract

Prior research has demonstrated cells exposed to silver nanoparticles (AgNPs) undergo endoplasmic reticulum (ER) stress leading to cellular apoptosis and toxicity, however, the fundamental mechanism underlying AgNP-induced ER stress is unknown. We hypothesize the biophysical interactions between AgNPs and adsorbed proteins lead to misfolded proteins to elicit an ER stress response. Our investigation examined rat aortic endothelial cells (RAEC) exposed to 20 or 100 nm AgNPs with or without a biocorona (BC) consisting of bovine serum albumin (BSA), high density lipoprotein (HDL) or fetal bovine serum (FBS) to form a complex BC. The presence of a BC consisting of BSA or FBS proteins significantly reduced uptake of 20 nm and 100 nm AgNPs in RAEC. Western blot analysis indicated robust activation of the IREα and PERK pathways in RAEC exposed to 20 nm despite the reduction in uptake by the presence of a BC. This was not observed for the 100 nm AgNPs. Hyperspectral darkfield microscopy qualitatively confirmed that the preformed BC was maintained following uptake by RAEC. Transmission electron microscopy demonstrated a size dependent effect on the sub-cellular localization of AgNPs. Overall, these results suggest that AgNP size, surface area and BC formation governs the induction of ER stress and alterations in intracellular trafficking.

Indexed as

Protein CoronaAdsorptionAnimalsApoptosisCells, CulturedCell SurvivalCircular DichroismEndoplasmic Reticulum StressEndothelial CellsLipoproteins, HDLMetal NanoparticlesMicroscopy, Electron, TransmissionOxidative StressRatsSerum Albumin, BovineSilverLipoproteins, HDLProtein CoronaSerum Albumin, BovineSilverAgNPER stressHyperspectral imagingNanoparticle toxicityNanotoxicologyProtein corona

Identifiers

PMID30529623
PMCPMC6331260
OpenAlexW2902208848

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.