ArticleThe Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation2019
Cell replacement in human lung bioengineering.
Article in The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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Who cites it
16 citing papers in PubMed, 36 citations in OpenAlex.
- Decellularized Rat Lung Extracellular Matrix as an In Vitro Platform for Canine Yolk Sac-Derived Endothelial Precursor Cells for Pulmonary Endothelium Reconstruction Studies.Bioengineering (Basel, Switzerland) · 2026Article
- Cell therapy strategies for organ regeneration.BMC medicine · 2026Review
- Advancements in lung regeneration: from bench to bedside.Journal of translational medicine · 2025Review
- Alveolar Organoids in Lung Disease Modeling.Biomolecules · 2024Review
- Biomimetic scaffold-based stem cell transplantation promotes lung regeneration.Bioengineering & translational medicine · 2023Article
- Technique for xenogeneic cross-circulation to support human donor lungs ex vivo.The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation · 2023Article
- Engineering Dynamic 3D Models of Lung.Advances in experimental medicine and biology · 2023Article
- Decellularized extracellular matrix scaffolds: Recent trends and emerging strategies in tissue engineering.Bioactive materials · 2022Review
- Imaging-guided bioreactor for de-epithelialization and long-term cultivation ofLab on a chip · 2022Article
- Homogeneous Distribution of Exogenous Cells onto De-epithelialized Rat Trachea via Instillation of Cell-Loaded Hydrogel.ACS biomaterials science & engineering · 2022Article
- Xenogeneic cross-circulation for extracorporeal recovery of injured human lungs.Nature medicine · 2020Article
- Multiday maintenance of extracorporeal lungs using cross-circulation with conscious swine.The Journal of thoracic and cardiovascular surgery · 2020Article
- Bioengineering the Blood-gas Barrier.Comprehensive Physiology · 2020Review
- Bioengineering of Pulmonary Epithelium With Preservation of the Vascular Niche.Frontiers in bioengineering and biotechnology · 2020Review
- Where We Stand: Lung Organotypic Living Systems That Emulate Human-Relevant Host-Environment/Pathogen Interactions.Frontiers in bioengineering and biotechnology · 2020Review
- Bioengineering approaches to organ preservation ex vivo.Experimental biology and medicine (Maywood, N.J.) · 2019Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
backgroundAs the number of patients with end-stage lung disease continues to rise, there is a growing need to increase the limited number of lungs available for transplantation. Unfortunately, attempts at engineering functional lung de novo have been unsuccessful, and artificial mechanical devices have limited utility as a bridge to transplant. This difficulty is largely due to the size and inherent complexity of the lung; however, recent advances in cell-based therapeutics offer a unique opportunity to enhance traditional tissue-engineering approaches with targeted site- and cell-specific strategies.
methodsHuman lungs considered unsuitable for transplantation were procured and supported using novel cannulation techniques and modified ex-vivo lung perfusion. Targeted lung regions were treated using intratracheal delivery of decellularization solution. Labeled mesenchymal stem cells or airway epithelial cells were then delivered into the lung and incubated for up to 6 hours.
resultsTissue samples were collected at regular time intervals and detailed histologic and immunohistochemical analyses were performed to evaluate the effectiveness of native cell removal and exogenous cell replacement. Regional decellularization resulted in the removal of airway epithelium with preservation of vascular endothelium and extracellular matrix proteins. After incubation, delivered cells were retained in the lung and showed homogeneous topographic distribution and flattened cellular morphology.
conclusionsOur findings suggest that targeted cell replacement in extracorporeal organs is feasible and may ultimately lead to chimeric organs suitable for transplantation or the development of in-situ interventions to treat or reverse disease, ultimately negating the need for transplantation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.