Evidence map›Paper›PMID 30529144›Full record

ArticleInsect biochemistry and molecular biology2019

Structural and functional characterization of an intradiol ring-cleavage dioxygenase from the polyphagous spider mite herbivore Tetranychus urticae Koch.

Caleb R Schlachter, Leily Daneshian, Jose Amaya, Vincent Klapper, Nicky Wybouw, Tomasz Borowski, Thomas Van Leeuwen, Vojislava Grbic, Miodrag Grbic, Thomas M Makris and 1 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Insect biochemistry and molecular biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.2field-weighted citation impact, top 51% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 24 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 4 institutions in 7 countries.

Caleb R SchlachterDepartment of Chemistry and Biochemistry, University of South Carolina, Columbia, SC, 29208, USA.
Leily DaneshianDepartment of Chemistry and Biochemistry, University of South Carolina, Columbia, SC, 29208, USA.
Jose AmayaDepartment of Chemistry and Biochemistry, University of South Carolina, Columbia, SC, 29208, USA.
Vincent KlapperDepartment of Chemistry and Biochemistry, University of South Carolina, Columbia, SC, 29208, USA.
Nicky WybouwInstitute for Biodiversity and Ecosystem Dynamics, University of Amsterdam, Science Park 904, 1098 XH, Amsterdam, the Netherlands; Department of Plants and Crops, Ghent University, Ghent, B-9000, Belgium.
Tomasz BorowskiJerzy Haber Institute of Catalysis and Surface Chemistry, Polish Academy of Sciences, 30-239, Krakow, Poland.
Thomas Van LeeuwenInstitute for Biodiversity and Ecosystem Dynamics, University of Amsterdam, Science Park 904, 1098 XH, Amsterdam, the Netherlands; Department of Plants and Crops, Ghent University, Ghent, B-9000, Belgium.
Vojislava GrbicDepartment of Biology, Western University, London, Ontario, N6A 5B7, Canada; University of La Rioja, Logrono, Spain.
Miodrag GrbicDepartment of Biology, Western University, London, Ontario, N6A 5B7, Canada; University of La Rioja, Logrono, Spain.
Thomas M MakrisDepartment of Chemistry and Biochemistry, University of South Carolina, Columbia, SC, 29208, USA.
Maksymilian ChruszczDepartment of Chemistry and Biochemistry, University of South Carolina, Columbia, SC, 29208, USA. Electronic address: chruszcz@mailbox.sc.edu.
University of South Carolina · USGhent University · BEWestern University · CAJerzy Haber Institute of Catalysis and Surface Chemistry, Polish Academy of Sciences · PL

Funding

Antigenic determinants of asthma-associated allergens for design of immunotherapy.R01AI077653 · NIAID · INDOOR BIOTECHNOLOGIES · PI MARTIN D. CHAPMAN, Maksymilian Chruszcz · 2009 to 2026
$9.5M
NIAID NIH HHS R01 AI077653
6 · The paper itself

Abstract

Genome analyses of the polyphagous spider mite herbivore Tetranychus urticae (two-spotted spider mite) revealed the presence of a set of 17 genes that code for secreted proteins belonging to the "intradiol dioxygenase-like" subgroup. Phylogenetic analyses indicate that this novel enzyme family has been acquired by horizontal gene transfer. In order to better understand the role of these proteins in T. urticae, we have structurally and functionally characterized one paralog (tetur07g02040). It was demonstrated that this protein is indeed an intradiol ring-cleavage dioxygenase, as the enzyme is able to cleave catechol between two hydroxyl-groups using atmospheric dioxygen. The enzyme was characterized functionally and structurally. The active site of the T. urticae enzyme contains an Fe

Indexed as

Gene Transfer, HorizontalAnimalsArthropod ProteinsDioxygenasesTetranychidaeArthropod ProteinsDioxygenasesHorizontal gene transferIntradiol ring-cleavage dioxygenasePlant-herbivore interactionstetur07g02040Two-spotted spider mite

Identifiers

PMID30529144
PMCPMC6768081
OpenAlexW2903168742

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.