ArticleMolecular metabolism2019
Preadipocytes of obese humans display gender-specific bioenergetic responses to glucose and insulin.
Article in Molecular metabolism, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Sex-dependent and compartment-specific macrophage accumulation associates with glomerular injury in BTBR ob/ob mice.Pflugers Archiv : European journal of physiology · 2026Article
- Sex-specific metabolic responses to high-fat diet in mice with NOX4 deficiency.Redox biology · 2025Article
- Article
- Redox dynamics in seeds ofFrontiers in plant science · 2024Article
- Adverse bioenergetic effects of N-acyl amino acids in human adipocytes overshadow beneficial mitochondrial uncoupling.Redox biology · 2023Article
- A single nuclei atlas of aging human abdominal subcutaneous white adipose tissue.Research square · 2023Article
- Glucose consumption of vascular cell types in culture: toward optimization of experimental conditions.American journal of physiology. Cell physiology · 2022Article
- Self-reported sleep efficiency and duration are associated with bioenergetic function in peripheral blood mononuclear cells (PBMCs) of adults.Mitochondrion · 2022Article
- From OCR and ECAR to energy: Perspectives on the design and interpretation of bioenergetics studies.The Journal of biological chemistry · 2021Review
- Sex differences in human adipose tissue gene expression and genetic regulation involve adipogenesis.Genome research · 2020Article
- TheBiomedicines · 2020Article
- Blood-based bioenergetics: An emerging translational and clinical tool.Molecular aspects of medicine · 2020Review
- On the role of macrophages in the control of adipocyte energy metabolism.Endocrine connections · 2019Review
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Authors and funding
12 authors.
Funding
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Abstract
BACKGROUND/
objectivesAlthough the prevalence of obesity and its associated metabolic disorders is increasing in both sexes, the clinical phenotype differs between men and women, highlighting the need for individual treatment options. Mitochondrial dysfunction in various tissues, including white adipose tissue (WAT), has been accepted as a key factor for obesity-associated comorbidities such as diabetes. Given higher expression of mitochondria-related genes in the WAT of women, we hypothesized that gender differences in the bioenergetic profile of white (pre-) adipocytes from obese (age- and BMI-matched) donors must exist. SUBJECTS/
methodsUsing Seahorse technology, we measured oxygen consumption rates (OCR) and extracellular acidification rates (ECAR) of (pre-)adipocytes from male (n = 10) and female (n = 10) deeply-phenotyped obese donors under hypo-, normo- and hyperglycemic (0, 5 and 25 mM glucose) and insulin-stimulated conditions. Additionally, expression levels (mRNA/protein) of mitochondria-related genes (e.g. UQCRC2) and glycolytic enzymes (e.g. PKM2) were determined.
resultsDissecting cellular OCR and ECAR into different functional modules revealed that preadipocytes from female donors show significantly higher mitochondrial to glycolytic activity (higher OCR/ECAR ratio, p = 0.036), which is supported by a higher ratio of UQCRC2 to PKM2 mRNA levels (p = 0.021). However, no major gender differences are detectable in in vitro differentiated adipocytes (e.g. OCR/ECAR, p = 0.248). Importantly, glucose and insulin suppress mitochondrial activity (i.e. ATP-linked respiration) significantly only in preadipocytes of female donors, reflecting their trends towards higher insulin sensitivity.
conclusionsCollectively, we show that preadipocytes, but not in vitro differentiated adipocytes, represent a model system to reveal gender differences with clinical importance for metabolic disease status. In particular preadipocytes of females maintain enhanced mitochondrial flexibility, as demonstrated by pronounced responses of ATP-linked respiration to glucose.
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