Evidence map›Paper›PMID 30527760›Full record

ArticleStem cell reports2019

Engineered Microenvironment for Manufacturing Human Pluripotent Stem Cell-Derived Vascular Smooth Muscle Cells.

Haishuang Lin, Xuefeng Qiu, Qian Du, Qiang Li, Ou Wang, Leonard Akert, Zhanqi Wang, Dirk Anderson, Kan Liu, Linxia Gu and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
1.4field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed, 39 citations in OpenAlex.

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  11. Alginate microfibers as therapeutic delivery scaffolds and tissue mimics.Experimental biology and medicine (Maywood, N.J.) · 2022
    Review
  12. Article
  13. Electrospun fibre diameter and its effects on vascular smooth muscle cells.Journal of materials science. Materials in medicine · 2021
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 3 institutions in 2 countries.

Haishuang LinDepartment of Chemical and Biomolecular Engineering, University of Nebraska-Lincoln, Lincoln, NE 68588, USA.
Xuefeng QiuDepartment of Cardiovascular Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
Qian DuDepartment of Biological Systems Engineering, University of Nebraska-Lincoln, Lincoln, NE 68588, USA.
Qiang LiDepartment of Chemical and Biomolecular Engineering, University of Nebraska-Lincoln, Lincoln, NE 68588, USA; Biomedical Engineering Program, University of Nebraska-Lincoln, Lincoln, NE 68588, USA.
Ou WangDepartment of Chemical and Biomolecular Engineering, University of Nebraska-Lincoln, Lincoln, NE 68588, USA; Biomedical Engineering Program, University of Nebraska-Lincoln, Lincoln, NE 68588, USA.
Leonard AkertDepartment of Chemical and Biomolecular Engineering, University of Nebraska-Lincoln, Lincoln, NE 68588, USA.
Zhanqi WangDepartment of Vascular Surgery, Beijing Anzhen Hospital of Capital Medical University, Beijing Institute of Heart, Lung, and Blood Vessel Diseases, Beijing 100029, China.
Dirk AndersonCenter for Biotechnology, University of Nebraska-Lincoln, Lincoln, NE 68588, USA.
Kan LiuDepartment of Biological Systems Engineering, University of Nebraska-Lincoln, Lincoln, NE 68588, USA.
Linxia GuDepartment of Mechanical and Materials Engineering, University of Nebraska-Lincoln, Lincoln, NE 68588, USA.
Chi ZhangDepartment of Biological Systems Engineering, University of Nebraska-Lincoln, Lincoln, NE 68588, USA.
Yuguo LeiDepartment of Chemical and Biomolecular Engineering, University of Nebraska-Lincoln, Lincoln, NE 68588, USA; Biomedical Engineering Program, University of Nebraska-Lincoln, Lincoln, NE 68588, USA; Mary and Dick Holland Regenerative Medicine Program, University of Nebraska Medical Center, Omaha, NE 68198, USA; Fred & Pamela Buffett Cancer Center, University of Nebraska Medical Center, Omaha, NE 68198, USA. Electronic address: ylei14@unl.edu.
University of Nebraska–Lincoln · USCapital Medical University · CNUnion Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Human pluripotent stem cell-derived vascular smooth muscle cells (hPSC-VSMCs) are of great value for disease modeling, drug screening, cell therapies, and tissue engineering. However, producing a high quantity of hPSC-VSMCs with current cell culture technologies remains very challenging. Here, we report a scalable method for manufacturing hPSC-VSMCs in alginate hydrogel microtubes (i.e., AlgTubes), which protect cells from hydrodynamic stresses and limit cell mass to <400 μm to ensure efficient mass transport. The tubes provide cells a friendly microenvironment, leading to extremely high culture efficiency. We have shown that hPSC-VSMCs can be generated in 10 days with high viability, high purity, and high yield (∼5.0 × 10

Indexed as

Stem Cell NicheAlginatesCell DifferentiationCells, CulturedCell SurvivalCellular Reprogramming TechniquesHumansHydrogelsInduced Pluripotent Stem CellsMuscle, Smooth, VascularMyocytes, Smooth MuscleTissue ScaffoldsAlginatesHydrogels3D culturealginate hydrogel tubehuman pluripotent stem cellsvascular smooth muscle cells

Identifiers

PMID30527760
PMCPMC6335449
OpenAlexW2903896036

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.