ArticleBiophysical journal2018
Single-Nanoparticle Tracking with Angstrom Localization Precision and Microsecond Time Resolution.
Article in Biophysical journal, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Neck region-microtubule interactions direct counterclockwise stepping of kinesin-1.Biophysical journal · 2026Article
- Metallic nanoparticles: from biosynthesis to biomedical applications, current scenarios and prospects.Nanoscale advances · 2026Review
- Meta-amplified dark-field interferometric scattering microscopy.Nature communications · 2026Article
- Article
- Rational engineering of DNA-nanoparticle motor with high speed and processivity comparable to motor proteins.Nature communications · 2025Article
- Visualizing Single V-ATPase Rotation Using Janus Nanoparticles.Nano letters · 2024Article
- Visualizing Single V-ATPase Rotation Using Janus Nanoparticles.bioRxiv : the preprint server for biology · 2024Article
- Deep learning-based size prediction for optical trapped nanoparticles and extracellular vesicles from limited bandwidth camera detection.Biomedical optics express · 2024Article
- Direct observation of stepping rotation of V-ATPase reveals rigid component in coupling between VProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Three-Dimensional Tracking of Tethered Particles for Probing Nanometer-Scale Single-Molecule Dynamics Using a Plasmonic Microscope.ACS sensors · 2021Article
- Micromirror Total Internal Reflection Microscopy for High-Performance Single Particle Tracking at Interfaces.ACS photonics · 2021Article
- Scattering-based Light Microscopy: From Metal Nanoparticles to Single Proteins.Chemical reviews · 2021Review
- Atomic scale displacements detected by optical image cross-correlation analysis and 3D printed marker arrays.Scientific reports · 2021Article
- Small stepping motion of processive dynein revealed by load-free high-speed single-particle tracking.Scientific reports · 2020Article
- Crystalline chitin hydrolase is a burnt-bridge Brownian motor.Biophysics and physicobiology · 2020Article
- Single-molecule analysis reveals rotational substeps and chemo-mechanical coupling scheme ofThe Journal of biological chemistry · 2019Article
- Damped White Noise Diffusion with Memory for Diffusing Microprobes in Ageing Fibrin Gels.Biophysical journal · 2019Article
- Insights into Kinesin-1 Stepping from Simulations and Tracking of Gold Nanoparticle-Labeled Motors.Biophysical journal · 2019Article
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7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gold nanoparticles (AuNPs) have been used as a contrast agent for optical imaging of various single biomolecules. Because AuNPs have high scattering efficiency without photobleaching, biomolecular dynamics have been observed with nanometer localization precision and sub-millisecond time resolution. To understand the working principle of biomolecular motors in greater detail, further improvement of the localization precision and time resolution is necessary. Here, we investigated the lower limit of localization precision achievable with AuNPs and the fundamental law, which determines the localization precision. We first used objective-lens-type total internal reflection dark-field microscopy to obtain a scattering signal from an isolated AuNP. The localization precision was inversely proportional to the square root of the photon number, which is consistent with theoretical estimation. The lower limit of precision for a 40 nm AuNP was ∼0.3 nm with 1 ms time resolution and was restricted by detector saturation. To achieve higher localization precision, we designed and constructed an annular illumination total internal reflection dark-field microscopy system with an axicon lens, which can illuminate the AuNPs at high laser intensity without damaging the objective lens. In addition, we used high image magnification to avoid detector saturation. Consequently, we achieved 1.3 Å localization precision for 40 nm AuNPs and 1.9 Å localization precision for 30 nm AuNPs at 1 ms time resolution. Furthermore, even at 33 μs time resolution, localization precisions at 5.4 Å for 40 nm AuNPs and at 1.7 nm for 30 nm AuNPs were achieved. We then observed motion of head of kinesin-1 labeled with AuNP at microsecond time resolution. Transition cycles of bound/unbound states and tethered diffusion of unbound head during stepping motion on microtubule were clearly captured with higher time resolution or smaller AuNP than those used in previous studies, indicating applicability to single-molecule imaging of biomolecular motors.
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