Evidence map›Paper›PMID 30527201›Full record

ArticleThe Kaohsiung journal of medical sciences2018

Computational modeling and functional characterization of a GgChi: A class III chitinase from corms of Gladiolus grandiflorus.

Maria Rafiq, Ashiq Hussain, Kausar Hussain Shah, Qamar Saeed, Muhammad Umair Sial, Zahid Ali, Friedrich Buck, Richard E Goodman, Binish Khaliq, Uzma Ishaq and 4 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Kaohsiung journal of medical sciences, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 46% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Expression and Refolding of the Plant Chitinase FromFrontiers in bioengineering and biotechnology · 2021
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 7 institutions in 4 countries.

Maria RafiqBotany Division, Institute of Pure and Applied Biology, Bahauddin Zakariya University, Multan, Pakistan.
Ashiq HussainTUM School of Life Sciences Weihenstephan, Technical University of Munich, Germany.
Kausar Hussain ShahBotany Division, Institute of Pure and Applied Biology, Bahauddin Zakariya University, Multan, Pakistan.
Qamar SaeedDepartment of Entomology, Bahauddin Zakariya University, Multan, Pakistan.
Muhammad Umair SialInstitute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, PR China.
Zahid AliDepartment of Biosciences, COMSATS University Islamabad, Park Road, Islamabad, Pakistan.
Friedrich BuckInstitute of Clinical Chemistry, University Medical Centre Hamburg-Eppendorf, Hamburg, Germany.
Richard E GoodmanFood Allergy Research and Resource Program, Department of Food Science & Technology, University of Nebraska-Lincoln, Lincoln, USA.
Binish KhaliqBotany Division, Institute of Pure and Applied Biology, Bahauddin Zakariya University, Multan, Pakistan.
Uzma IshaqDepartment of Botany, The Women University, Multan, Pakistan.
Mirza Ahsen BaigBotany Division, Institute of Pure and Applied Biology, Bahauddin Zakariya University, Multan, Pakistan.
Aisha MunawarDepartment of Chemistry, Faculty of Natural Sciences, Humanities & Islamic Studies, University of Engineering and Technology, Lahore, Pakistan.
Seema MahmoodBotany Division, Institute of Pure and Applied Biology, Bahauddin Zakariya University, Multan, Pakistan.
Ahmed AkremBotany Division, Institute of Pure and Applied Biology, Bahauddin Zakariya University, Multan, Pakistan. Electronic address: ahmedakrem@bzu.edu.pk.
Bahauddin Zakariya University · PKUniversity of Nebraska–Lincoln · USCOMSATS University Islamabad · PKInstitute of Plant Protection · CNTechnical University of Munich · DEUniversität Hamburg · DEUniversity of Engineering and Technology Lahore · PK

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The present study describes the predicted model and functional characterization of an endochitinase (30 kDa) from corms of Gladiolus grandiflorus. ESI-QTOF-MS generated peptide showed 96% sequence homology with family 18, Class III acidic endochitinase of Gladiolus gandavensis. Purified G. grandiflorus chitinase (GgChi) hydrolyzed 4-methylumbelliferyl β-d-N,N',N''-triacetylchitotriose substrate showing specific endochitinase activity. Since no structural details of GgChi were available in the Protein Data Bank (PDB), a homology model was predicted using the coordinate information of Crocus vernus chitinase (PDB ID: 3SIM). Ramachandran plot indicated 84.5% in most favored region, 14.8% in additional and 0.6% in generously allowed region while no residue in disallowed region. The predicted structure indicated a highly conserved (β/α)

Indexed as

Models, MolecularAmino Acid SequenceAnimalsAnti-Bacterial AgentsAntifungal AgentsBacteriaChitinasesDatabases, ProteinEnzyme AssaysFungiHemipteraInsecticidesIridaceaeMicrobial Sensitivity TestsPlant ProteinsSpectrometry, Mass, Electrospray IonizationAnti-Bacterial AgentsAntifungal Agentschitinase, class IIIChitinasesInsecticidesPlant ProteinsAntibacterialAntifungalEndochitinaseGladiolus grandiflorusTIM barrel

Identifiers

PMID30527201
PMCPMC11915627
OpenAlexW2897789572

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.