ArticleBMC plant biology2018
Cloning and characterization of norbelladine synthase catalyzing the first committed reaction in Amaryllidaceae alkaloid biosynthesis.
Article in BMC plant biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- A chromosome-level genome assembly of Lycoris radiata reveals the evolutionary origin of Amaryllidaceae alkaloids and elucidates the complete galanthamine biosynthetic pathway.Plant communications · 2026Article
- Amaryllidaceae Alkaloids and Isoquinoline Alkaloids: A Perspective on Historical Approaches to Pathway Elucidation.Plants (Basel, Switzerland) · 2026Review
- Biosynthesis of benzylisoquinoline alkaloids and its evolution in plants.Plant communications · 2026Review
- Protection of naringenin chalcone by a pathogenesis-related 10 protein promotes flavonoid biosynthesis in Marchantia polymorpha.The New phytologist · 2025Article
- Elucidating the enzyme network driving Amaryllidaceae alkaloids biosynthesis in Leucojum aestivum.Plant biotechnology journal · 2025Article
- Navigating Amaryllidaceae alkaloids: bridging gaps and charting biosynthetic territories.Journal of experimental botany · 2025Review
- Multiple NADPH-cytochrome P450 reductases from Lycoris radiata involved in Amaryllidaceae alkaloids biosynthesis.Plant molecular biology · 2024Article
- Synthesis, Characterization, and Biological Evaluation ofMolecules (Basel, Switzerland) · 2024Article
- Biosensor and machine learning-aided engineering of an amaryllidaceae enzyme.Nature communications · 2024Article
- Genome-wide identification of rubber tree pathogenesis-related 10 (PR-10) proteins with biological relevance to plant defense.Scientific reports · 2024Article
- Functional characterization of CYP96T1-like cytochrome P450 fromFrontiers in plant science · 2024Article
- Emerging functions within the enzyme families of plant alkaloid biosynthesis.Phytochemistry reviews : proceedings of the Phytochemical Society of Europe · 2023Article
- Small RNAs and Karma methylation in Elaeis guineensis mother palms are linked to high clonal mantling.Plant molecular biology · 2023Article
- SWATH-MS-Based Proteomics Reveals the Regulatory Metabolism of Amaryllidaceae Alkaloids in ThreeInternational journal of molecular sciences · 2023Article
- Characterization of norbelladine synthase and noroxomaritidine/norcraugsodine reductase reveals a novel catalytic route for the biosynthesis of Amaryllidaceae alkaloids including the Alzheimer's drug galanthamine.Frontiers in plant science · 2023Article
- Chemical Synthesis and Biological Activities of Amaryllidaceae Alkaloid Norbelladine Derivatives and Precursors.Molecules (Basel, Switzerland) · 2022Article
- Genome-Wide Transcriptomic Analysis of the Effects of Infection with the Hemibiotrophic FungusPlants (Basel, Switzerland) · 2022Article
- Biotechnological Approaches to Optimize the Production of Amaryllidaceae Alkaloids.Biomolecules · 2022Review
- Transcriptome analysis of Leucojum aestivum and identification of genes involved in norbelladine biosynthesis.Planta · 2022Article
- An ATP-Binding Cassette Transporter, LaABCB11, Contributes to Alkaloid Transport inInternational journal of molecular sciences · 2021Article
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Abstract
backgroundAmaryllidaceae alkaloids (AAs) are a large group of plant-specialized metabolites displaying an array of biological and pharmacological properties. Previous investigations on AA biosynthesis have revealed that all AAs share a common precursor, norbelladine, presumably synthesized by an enzyme catalyzing a Mannich reaction involving the condensation of tyramine and 3,4-dihydroxybenzaldehyde. Similar reactions have been reported. Specifically, norcoclaurine synthase (NCS) which catalyzes the condensation of dopamine and 4-hydroxyphenylacetaldehyde as the first step in benzylisoquinoline alkaloid biosynthesis.
resultsWith the availability of wild daffodil (Narcissus pseudonarcissus) database, a transcriptome-mining search was performed for NCS orthologs. A candidate gene sequence was identified and named norbelladine synthase (NBS). NpNBS encodes for a small protein of 19 kDa with an anticipated pI of 5.5. Phylogenetic analysis showed that NpNBS belongs to a unique clade of PR10/Bet v1 proteins and shared 41% amino acid identity to opium poppy NCS1. Expression of NpNBS cDNA in Escherichia coli produced a recombinant enzyme able to condense tyramine and 3,4-DHBA into norbelladine as determined by high-resolution tandem mass spectrometry.
conclusionsHere, we describe a novel enzyme catalyzing the first committed step of AA biosynthesis, which will facilitate the establishment of metabolic engineering and synthetic biology platforms for the production of AAs.
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