Evidence map›Paper›PMID 30524943›Full record

ArticleFEBS open bio2018

Quantitative analysis of sensitivity to a Wnt3a gradient in determination of the pole-to-pole axis of mitotic cells by using a microfluidic device.

Takumi Hiraiwa, Yuichiro Nakai, Takahiro G Yamada, Ryuichi Tanimoto, Hiroshi Kimura, Yoshinori Matsumoto, Norihisa Miki, Noriko Hiroi, Akira Funahashi

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in FEBS open bio, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 90% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Takumi HiraiwaDepartment of Biosciences and Informatics Keio University Yokohama Japan.
Yuichiro NakaiDepartment of Biosciences and Informatics Keio University Yokohama Japan.
Takahiro G YamadaDepartment of Biosciences and Informatics Keio University Yokohama Japan.
Ryuichi TanimotoDepartment of Biosciences and Informatics Keio University Yokohama Japan.
Hiroshi KimuraDepartment of Mechanical Engineering Tokai University Hiratsuka Japan.
Yoshinori MatsumotoDepartment of Applied Physics and Physico-Informatics Keio University Yokohama Japan.
Norihisa MikiDepartment of Mechanical Engineering Keio University Yokohama Japan.
Noriko HiroiDepartment of Biosciences and Informatics Keio University Yokohama Japan.ORCID 0000-0002-0214-373X
Akira FunahashiDepartment of Biosciences and Informatics Keio University Yokohama Japan.
Keio University · JPTokai University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Proper determination of the cell division axis is essential during development. Wnt3a is a known regulator of the cell division axis; however, the sensitivity of cells to Wnt3a signalling and its role in determining the cell division axis have not been measured to date. To address this gap, we took advantage of the asymmetric distribution of outer dense fibre 2 (ODF2/cenexin) proteins on centrosomes in dividing cells. To precisely quantify the sensitivity of cells to Wnt3a signalling, we developed a microfluidic cell culture device, which can produce a quantitative gradient of signalling molecules. We confirmed that mitotic SH-SY5Y neuroblastoma cells could detect a 2.5 ~ 5 × 10

Indexed as

microfluidicsneuroblastomapole‐to‐pole axisquantitative gradient ODF2Wnt3a

Identifiers

PMID30524943
PMCPMC6275273
OpenAlexW2891583827

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.