Evidence map›Paper›PMID 30521678›Full record

ArticleChembiochem : a European journal of chemical biology2019

A Simple DNAzyme-Based Fluorescent Assay for Klebsiella pneumoniae.

M Monsur Ali, Anatoly Slepenkin, Ellena Peterson, Weian Zhao

Abstract read
In one paragraph

Article in Chembiochem : a European journal of chemical biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed.

  1. Article
  2. Aptamer and DNAzyme Based Colorimetric Biosensors for Pathogen Detection.Angewandte Chemie (International ed. in English) · 2025
    Review
  3. Enhanced Detection ofBiosensors · 2024
    Article
  4. A DNAzymes-in-droplets assay forChemical science · 2024
    Article
  5. Article
  6. Review
  7. Review
  8. Biosensing with DNAzymes.Chemical Society reviews · 2021
    Review
  9. Rapid detection ofFood science & nutrition · 2021
    Article
  10. Review
  11. DNAmoreDB, a database of DNAzymes.Nucleic acids research · 2021
    Article
  12. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

M Monsur AliBiointerfaces Institute, McMaster University, 1280 Main St. W., Hamilton, ON, L8S 4K1, Canada.ORCID 0000-0002-6308-2205
Anatoly SlepenkinDepartment of Pathology and Laboratory Medicine, University of California, 1001 Health Sciences Road, Irvine, CA, 92697, USA.
Ellena PetersonDepartment of Pathology and Laboratory Medicine, University of California, 1001 Health Sciences Road, Irvine, CA, 92697, USA.
Weian ZhaoDepartment of Pharmaceutical Sciences, Sue and Bill Gross Stem Cell Research Center, Edwards Lifesciences Center for Advanced Cardiovascular Technology and, The Department of Biomedical Engineering, University of California-Irvine, 845 Health Sciences Road, Irvine, CA, 92697, USA.

Funding

University of California Health Participation in the National COVID Cohort Collaborative (N3C)UL1TR001414 · NCATS · UNIVERSITY OF CALIFORNIA-IRVINE · PI COOPER, DAN M, VILAIN, ERIC J. · 2015 to 2023
$35.1M
Integrated Comprehensive Droplet Digital Detection (IC 3D) system for rapid detection of bacteria andantimicrobial resistanceR01AI117061 · NIAID · UNIVERSITY OF CALIFORNIA-IRVINE · PI ZHAO, WEIAN · 2015 to 2019
$5.1M
NCATS NIH HHS UL1 TR001414NIAID NIH HHS R01 AI117061
6 · The paper itself

Abstract

Pathogenic bacteria pose a serious threat to public health, and the rapid and cost-effective detection of such bacteria remains a major challenge. Herein, we present a DNAzyme-based fluorescent paper sensor for Klebsiella pneumoniae. The DNAzyme was generated by an in vitro selection technique to cleave a fluorogenic DNA-RNA chimeric substrate in the presence of K. pneumoniae. The DNAzyme was printed on a paper substrate in a 96-well format to serve as mix-and-read fluorescent assay that exhibits a limit of detection (LOD) 10

Indexed as

Bacterial Typing TechniquesDNA, CatalyticFluorescent DyesFluorometryGene LibraryKlebsiella pneumoniaeLimit of DetectionPaperDNA, CatalyticFluorescent DyesDNAzymesfluorescenceKlebsiella pneumoniaepaper sensorpathogenic bacteria

Identifiers

PMID30521678
PMCPMC6692177

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.