Evidence map›Paper›PMID 30520207›Full record

ArticleChembiochem : a European journal of chemical biology2019

Pull-Down of Metalloproteins in Their Native States by Using Desthiobiotin-Based Probes.

Chinh Ngo, Radhika Mehta, Kanchan Aggarwal, Audrey G Fikes, Ines C Santos, Sylvester M Greer, Emily L Que

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Chembiochem : a European journal of chemical biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 40% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Proteomic strategies to interrogate the Fe-S proteome.Biochimica et biophysica acta. Molecular cell research · 2024
    Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Chinh NgoDepartment of Chemistry, University of Texas at Austin, 105 E 24th St. Stop A5300, Austin, TX, 78712, USA.ORCID 0000-0002-0152-3458
Radhika MehtaDepartment of Chemistry, University of Texas at Austin, 105 E 24th St. Stop A5300, Austin, TX, 78712, USA.ORCID 0000-0002-8677-110X
Kanchan AggarwalDepartment of Chemistry, University of Texas at Austin, 105 E 24th St. Stop A5300, Austin, TX, 78712, USA.ORCID 0000-0001-9927-6098
Audrey G FikesDepartment of Chemistry, University of Texas at Austin, 105 E 24th St. Stop A5300, Austin, TX, 78712, USA.ORCID 0000-0003-4034-6260
Ines C SantosDepartment of Chemistry, University of Texas at Austin, 105 E 24th St. Stop A5300, Austin, TX, 78712, USA.ORCID 0000-0002-4609-7085
Sylvester M GreerDepartment of Chemistry, University of Texas at Austin, 105 E 24th St. Stop A5300, Austin, TX, 78712, USA.
Emily L QueDepartment of Chemistry, University of Texas at Austin, 105 E 24th St. Stop A5300, Austin, TX, 78712, USA.ORCID 0000-0001-6604-3052
The University of Texas at Austin · US

Funding

UVPD Mass Spectrometry of Protein ComplexesR01GM121714 · NIGMS · UNIVERSITY OF TEXAS AT AUSTIN · PI BRODBELT, JENNIFER S. · 2017 to 2020
$1.4M
Bruker AVANCE III HD 500 MHz NMRS10OD021508 · OD · UNIVERSITY OF TEXAS AT AUSTIN · PI SESSLER, JONATHAN L · 2016 to 2016
$529k
NIGMS NIH HHS R01 GM121714NIH HHS S10 OD021508
6 · The paper itself

Abstract

One-third of all proteins are estimated to require metals for structural stability and/or catalytic activity. Desthiobiotin probes containing metal binding groups can be used to capture metalloproteins with exposed active-site metals under mild conditions so as to prevent changes in metallation state. The proof-of-concept was demonstrated with carbonic anhydrase (CA), an open active site, Zn

Indexed as

BiotinCarbonic AnhydrasesHumansMetalloproteinsMolecular ProbesProtein ConformationSulfonamidesBiotinCarbonic AnhydrasesdesthiobiotinMetalloproteinsMolecular ProbesSulfonamidescarbonic anhydrasedesthiobiotinmetalloproteinsproteomicssulfonamide

Identifiers

PMID30520207
PMCPMC6530555
OpenAlexW2903306994

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.