ArticleAnalytical and bioanalytical chemistry2019
A validated workflow for drug detection in oral fluid by non-targeted liquid chromatography-tandem mass spectrometry.
Article in Analytical and bioanalytical chemistry, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 33 citations in OpenAlex.
- Insights into the human metabolism of hexahydrocannabinol by non-targeted liquid chromatography-high-resolution tandem mass spectrometry.Journal of analytical toxicology · 2024Article
- A critical review of workplace drug testing methods for old and new psychoactive substances: Gaps, advances, and perspectives.Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society · 2024Article
- Interpol review of toxicology 2019-2022.Forensic science international. Synergy · 2023Review
- Analysis of 2,5-dimethoxy-amphetamines and 2,5-dimethoxy-phenethylamines aiming their determination in biological matrices: a review.Forensic toxicology · 2023Review
- Stability of drugs of abuse in synthetic oral fluid investigated using a simple "dilute and inject" method of analysis.Drug testing and analysis · 2022Article
- Monomer Release from Dental Resins: The Current Status on Study Setup, Detection and Quantification for In Vitro Testing.Polymers · 2022Review
- In silico deconjugation of glucuronide conjugates enhances tandem mass spectra library annotation of human samples.Analytical and bioanalytical chemistry · 2022Article
- U-47700 and Its Analogs: Non-Fentanyl Synthetic Opioids Impacting the Recreational Drug Market.Brain sciences · 2020Review
- Targeted Metabolomic Profiling of Total Fatty Acids in Human Plasma by Liquid Chromatography-Tandem Mass Spectrometry.Metabolites · 2020Article
- Thermographic detection and quantification of THC in oral fluid at unprecedented low concentrations.Biomedical optics express · 2020Article
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Oral fluid is recognized as an important specimen for drug testing. Common applications are monitoring in substance abuse treatment programs, therapeutic drug monitoring, pain management, workplace drug testing, clinical toxicology, and driving under the influence of drugs (DRUID). In this study, we demonstrate that non-targeted LC-MS/MS with subsequent compound identification by tandem mass spectral library search is a valuable tool for comprehensive detection and confirmation of drugs in oral fluid samples. The workflow developed involves solid-phase extraction and chromatographic separation on reversed phase materials. Mass spectrometric detection is accomplished on a quadrupole-quadrupole-time-of-flight instrument operated with data-dependent acquisition control. The workflow was optimized for 500 μl of neat oral fluid collected with the Greiner Bio-One saliva collection system. The fitness of the developed method was tested and proven by analyzing blank and spiked samples as well as 59 authentic patient samples. We could demonstrate that compounds with logP values in the range 0.5-5.5 are efficiently detected at low nanograms per milliliter concentrations. The true positive and true negative rates of automated library search were equal or close to 100%. The beauty of the non-targeted LC-MS/MS approach is the ability to detect compounds hardly included in routinely applied targeted assays, and this was demonstrated by detecting the synthetic opioid U-47700 in two patient samples. Graphical abstract ᅟ.
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