ArticleFrontiers in medicine2018
Nanoscale Imaging of Kidney Glomeruli Using Expansion Pathology.
Article in Frontiers in medicine, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- In vitro evaluation of microfluidic WS6-loaded Eudragit nanoparticles for improving insulin-producing cell differentiation.PloS one · 2026Article
- Optical super-resolution histology of formalin-fixed paraffin-embedded tissue samples: challenges and opportunities.Nature communications · 2025Review
- BOOST: a robust ten-fold expansion method on hour-scale.Nature communications · 2025Article
- Ultrastructure expansion microscopy (U-ExM) of mouse and human kidneys for analysis of subcellular structures.Cytoskeleton (Hoboken, N.J.) · 2024Article
- Multiplexed spatial transcriptomics methods and the application of expansion microscopy.Frontiers in cell and developmental biology · 2024Review
- Identification and validation of a DNA methylation-driven gene-based prognostic model for clear cell renal cell carcinoma.BMC genomics · 2023Article
- Expansion Microscopy-based imaging for visualization of mitochondria in Drosophila ovarian germline stem cells.FEBS open bio · 2022Article
- Characterization of nanosensitive multifractality in submicron scale tissue morphology and its alteration in tumor progression.Journal of biomedical optics · 2021Article
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Authors and funding
2 authors.
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Abstract
Kidney glomerular diseases, such as the minimal change disease (MCD) and focal segmental glomerulosclerosis (FSGS), and other nephrotic syndromes, are typically diagnosed or confirmed via electron microscopy. Although optical microscopy has been a vital tool to examine clinical specimens for diagnoses in pathology for decades, the optical resolution is constricted by the physical diffraction limit of the optical microscope, which prevents high-resolution investigation of subcellular anatomy, such as of the podocyte tertiary foot processes. Here, we describe a simple, fast, and inexpensive protocol for nanoscale optical imaging of kidney glomeruli. The protocol is based on Expansion Pathology (ExPath), a new principle of microscopy that overcomes optical diffraction limit by chemically embedding specimens into a swellable polymer and physically expanding it homogenously prior to imaging. Our method uses only commercially available reagents, a conventional fluorescence microscope and it can be applied to both fixed-frozen or formalin-fixed paraffin embedded (FFPE) tissue sections. It requires minimal operative experience in a wet lab, optical microscopy and imaging processing. Finally, we also discuss challenges, limitations and prospective applications for ExPath-based imaging of glomeruli.
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Registered trials
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